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Series GSE112118 Query DataSets for GSE112118
Status Public on Jun 18, 2019
Title RNASeq of total RNA isolated from self-assembling co-cultures of primary human hepatocytes (SACC-PHHs) mono-infected with HBV, co-infected with HBV/HDV, or uninfected at 8 and 28 days post-infection
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we examined the host response relative of SACC-PHHs infected with either hepatitis B virus (HBV) alone or both HBV/hepatitis delta virus (HDV) co-infection compared to non-infected controls.
 
Overall design SACC-PHHs were generated with PHHs from either a single human donor or mixed donors (in total, there were five donors) and co-cultured with 3T3J mouse non-parenchymal cells. These cultures can be persistently infected for up to 1-1.5 months post-challenge and exhibit a transcriptomic profile similar to what's observed in the 3D context of the liver. Note that not all donors and conditions have the same number of replicates.
 
Contributor(s) Winer BY, Gaska JM, Lipkowitz G, Bram Y, Parekh A, Parsons L, Leach R, Jindal R, Cho CH, Shrirao A, Novik E, Ploss A
Citation(s) 31206195
Submission date Mar 20, 2018
Last update date Sep 20, 2019
Contact name Alexander Ploss
E-mail(s) aploss@princeton.edu
Phone 609-258-7128
Organization name Princeton University
Department Molecular Biology
Lab 110 Lewis Thomas Laboratory
Street address Washington Road
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Platforms (1)
GPL22245 Illumina HiSeq 2500 (Homo sapiens; Mus musculus)
Samples (44)
GSM3058085 Donor HU1007 (AKA Sample 1) HBV mono-infected for 8 days
GSM3058086 Donor HU1007 (AKA Sample 2) HBV mono-infected for 8 days
GSM3058087 Donor HU1007 (AKA Sample 3) HBV mono-infected for 8 days
Relations
BioProject PRJNA439332
SRA SRP136132

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112118_RAW.tar 18.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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