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Status |
Public on Aug 28, 2018 |
Title |
Role of SmcHD1 in the establishment of the epigenetic states required for the maintenance of X chromosome inactivation [RNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
It has been shown that functional deficiency of SmcHD1, a noncanonical member of SMC family proteins, results in derepression of X-inactivated genes in postimplantation female mouse embryos. In this study, we found that derepression of X-inactivated genes accompanied a local reduction in the enrichment of H3K27me3 in mouse embryonic fibroblasts (MEFs) prepared from female fetuses deficient for SmcHD1.
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Overall design |
Allele-specific RNA-seq analysis of mouse embryonic fibroblasts (MEFs) prepared from female fetuses deficient for SmcHD1. The MEFs were obtained from B6-JF1 F1 males crossed with B6 females carrying Xist-deltaA mutation.
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Contributor(s) |
Sakakibara Y, Nagao K, Obuse C, Sado T |
Citation(s) |
30126901 |
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Submission date |
Mar 20, 2018 |
Last update date |
Sep 12, 2018 |
Contact name |
Koji Nagao |
E-mail(s) |
nagao@bio.sci.osaka-u.ac.jp
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Organization name |
Osaka University
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Department |
Graduate School of Science
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Lab |
Obuse Lab.
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Street address |
1-1 Machikaneyama
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City |
Toyonaka |
State/province |
Osaka |
ZIP/Postal code |
560 0043 |
Country |
Japan |
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Platforms (1) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE112097 |
Role of SmcHD1 in the establishment of the epigenetic states required for the maintenance of X chromosome inactivation |
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Relations |
BioProject |
PRJNA439360 |
SRA |
SRP136200 |