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Series GSE111976 Query DataSets for GSE111976
Status Public on Jun 29, 2020
Title Single cell RNA-seq analysis on human endometrium across the natural menstrual cycle
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To unbiasedly and systematically characterize endometrial transformation across the human menstrual cycle in preparation for embryo implantation, we analyzed the transcriptomic transformation of human endometrium at single cell resolution, dissecting multidimensional cellular heterogeneity of the tissue across the entire natural menstrual cycle.
Overall design We collected single cell transcriptome profile of endometrium biopsies sampled from 19 healthy and fertile females, at 4-27 days of their menstrual cycle.

[10x dataset] - collected to validate main findings drawn from the original C1 dataset of this study. In this dataset, we collected additional data from 10 endometrial biopsies using the 10x Chromium system. Importantly, we collected both C1 and 10x data for two biopsies, one from mid-secretory phase and the other early-secretory. They served as anchors for a direct comparison between the two datasets, and were referred as ?anchor biopsies? in the manuscript of this study. Biopsies in the 10x dataset were obtained following the same protocol used for the C1 dataset, as described in the manuscript.
Contributor(s) Wang W, Isakova A, Vilella F, Alama P, Moreno I, Pan W, Simon C, Quake SR
Citation(s) 32929266, 34329437
Submission date Mar 17, 2018
Last update date Sep 08, 2021
Contact name Wanxin Wang
Phone 4134373134
Organization name Stanford Univeristy
Lab Wanxin Wang
Street address James H Clark Center, 318 Campus Drive, room E300
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2158)
GSM3045927 1772158101_C01_A3
GSM3045928 1772158101_C03_A1
GSM3045929 1772158101_C04_A9
BioProject PRJNA438770
SRA SRP135922

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Supplementary file Size Download File type/resource
GSE111976_README.txt 1.3 Kb (ftp)(http) TXT
GSE111976_ct.csv.gz 12.1 Mb (ftp)(http) CSV
GSE111976_ct_endo_10x.rds.gz 408.3 Mb (ftp)(http) RDS
GSE111976_summary_10x_day_donor_ctype.csv.gz 579.5 Kb (ftp)(http) CSV
GSE111976_summary_10x_donor_phase.csv.gz 159 b (ftp)(http) CSV
GSE111976_summary_C1_day_donor_ctype.csv.gz 16.9 Kb (ftp)(http) CSV
GSE111976_summary_C1_donor_phase.csv.gz 253 b (ftp)(http) CSV
GSE111976_umap_endo_10x.csv.gz 1.1 Mb (ftp)(http) CSV
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Processed data are available on Series record

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