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| Status |
Public on Mar 14, 2018 |
| Title |
Gene-expression analysis of human embryonic stem cell (HESC-)- derived rosette-forming neural progenitor cells (NPCs) in the presence of lysophosphatidic acid (LPA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
As part of our study on the effect of LPA on HESC-derived NPCs, we have employed whole genome microarray analysis to identify genes which were up- and downregulated in the presence of LPA compared to non-LPA-treated control-NPCs and which might be involved in the expansion of the apical domain of NPCs and large rosette formation that we observe after LPA-treatment.
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| Overall design |
Gene-expression of NPCs at rosette passaging number 2 (RP2) that were cultured in plastic 24-well plates and exposed for 2 h, 10 h and 24 h to 0.5 ml N2B27 + FGF2/EGF medium which was initially supplemented with 15 µM was performed. Experiments were performed in triplicates.
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| Contributor(s) |
Medelnik J |
| Citation(s) |
29779899 |
| |
| Submission date |
Mar 08, 2018 |
| Last update date |
Jun 21, 2018 |
| Contact name |
Jan-Philip Medelnik |
| E-mail(s) |
jan.medelnik@imp.ac.at
|
| Organization name |
Research Institute of Molecular Pathology (IMP)
|
| Lab |
Tanaka
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (18)
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| GSM3034738 |
control_[noLPA]_replicate1 (2h time point) |
| GSM3034739 |
LPA_[25uM]_replicate1 (2h time point) |
| GSM3034740 |
control_[noLPA]_replicate2 (2h time point) |
| GSM3034741 |
LPA_[25uM]_replicate2 (2h time point) |
| GSM3034742 |
control_[noLPA]_replicate3 (2h time point) |
| GSM3034743 |
LPA_[25uM]_replicate3 (2h time point) |
| GSM3034744 |
control_[noLPA]_replicate1 (10h time point) |
| GSM3034745 |
LPA_[25uM]_replicate1 (10h time point) |
| GSM3034746 |
control_[noLPA]_replicate2 (10h time point) |
| GSM3034747 |
LPA_[25uM]_replicate2 (10h time point) |
| GSM3034748 |
control[noLPA]_replicate3 (10h time point) |
| GSM3034749 |
LPA_[25uM]_replicate3 (10h time point) |
| GSM3034750 |
control_[noLPA]_replicate1 (24h time point) |
| GSM3034751 |
LPA_[25uM]_replicate1 (24h time point) |
| GSM3034752 |
control_[noLPA]_replicate2 (24h time point) |
| GSM3034753 |
LPA_[25uM]_replicate2 (24h time point) |
| GSM3034754 |
control_[noLPA]_replicate3 (24h time point) |
| GSM3034755 |
LPA_[25uM]_replicate3 (24h time point) |
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| Relations |
| BioProject |
PRJNA437487 |
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