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Series GSE111429 Query DataSets for GSE111429
Status Public on Mar 01, 2019
Title Identification of Zeb1 as a single marker for prostate stem cells in the basal cell compartment
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The prostate basal cell compartment is postulated to contain stem/progenitors due to its resistance to castration, capability to differentiation into basal, luminal and neuroendocrine cell lineages of prostate epithelium, and susceptibility to oncogenic transformation. However, the heterogeneity and the interrelationship among different cell subpopulations within prostate basal cells remain largely unknow. Here we find that the core epithelial-to-mesenchymal transition (EMT) inducer Zeb is exclusively expressed in a prostate basal cell subpopulation. The Zeb1+ basal cells are resistant against androgen deprivation, possess greater efficiency to produce prostate spheroids in vitro, undergo self-renewal, and can generate functional prostate with all three cell lineages in vivo at the single cell level. Utilizing unbiased single cell transcriptomic analysis of over 9000 mouse prostate basal cells, we find that Zeb1+ basal cell subset shares gene expression profile with both epithelial and mesenchymal cells and stands out uniquely among all the cell clusters. Pseudotemporal reconstruction revealed three cell lineage trajectories through which the Zeb1+ basal cell subset gives rise to differentiated basal cells, and androgen dependent or independent intermediate cells. at the starting point in the developmental trajectories of cell clusters in prostate basal epithelium. In addition, Zeb1 positive basal cells can be detected in human prostate samples. Our data demonstrate that these Zeb1+ cells are bona fide PSCs in the basal cell compartment. Identification of the prostate stem cell (PSC) and its differentiation path is crucial to advance our understanding of prostate development and tumorigenesis.
Overall design Exploration of transcriptional differences and interations among different subpopulations within prostate basal cells
Contributor(s) Wang X, Zhu HH
Citation(s) 32024836
Submission date Mar 05, 2018
Last update date Feb 18, 2020
Contact name Xue Wang
Organization name SJTU CHINA
Street address Huashan Rd. 1940, build 3. Shanghai,
City Shanghai
State/province Shanghai China
ZIP/Postal code 200017
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (1)
GSM3030643 RJ-S1
BioProject PRJNA436984
SRA SRP133953

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Supplementary file Size Download File type/resource
GSE111429_Data1.Epi.Clusters.DGEs.Markers.xlsx 236.8 Kb (ftp)(http) XLSX
GSE111429_RAW.tar 80.0 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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