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Series GSE111399 Query DataSets for GSE111399
Status Public on Jun 05, 2018
Title Characteristics of differentiated newly identified adipose precursor cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have identified proliferative cells in adipose tissue expressing adipocyte specific genes, which were named small proliferative adipocytes (SPA). Comparison of gene expression among stromal vascular cells (SVC), SPA and mature adipocyte (MA) revealed that adipocyte specific genes and several neuronal genes were increased in the order of SVC < SPA < MWA. However, proliferin 2 (PLF) was detected only in SPA. We found that SPA changed to lipid laden cells by incubation with differentiation medium containing insulin/dexamethasone/IBMX. In this study, we investigated the gene expression of differentiated SPA (SPAD) comparing with differentiated SVC (SVCD). Microarrays and real time PCR revealed that uncoupling protein 1 (UCP1) and PLF were strongly expressed in D-SPA. These data suggested that SPA might be able to differentiate into beige cells.
Overall design Microarray was performed to investigate the characteristics of differentiated SPA (SPAD). The results were confirmed with real time PCR and immunocytochemical study.
Contributor(s) Kajita K, Taguchi K
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Submission date Mar 05, 2018
Last update date Jun 06, 2018
Contact name Shigeo Takashima
Organization name Gifu University
Department Life Science Research Center
Street address 1-1 Yanagido
City Gifu city
State/province Gifu
ZIP/Postal code 501-1194
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (2)
GSM3030102 Differentiated small proliferative adipocytes
GSM3030103 Differentiated stromal viscular cells
BioProject PRJNA436915

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111399_RAW.tar 4.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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