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Series GSE111352 Query DataSets for GSE111352
Status Public on Feb 08, 2021
Title S1PR3–G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation [MCF-7 RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cancer tissues possess less glucose than surrounding normal tissue, because cancer cells predominantly produce energy by glycolysis than oxidative phosphorylation even in the presence of an adequate oxygen supply (Warburg effect). We found a novel compound ALESIA which causes apoptosis of malignant cells at a low glucose condition from an original phenotypic screening. Identified target molecule of ALESIA was Sphingosine-1-phosphate receptor 3 (S1PR3). Being different from the natural ligand, ALESIA selectively stimulated S1PR3-G12 coupling to S1PR3 without suppression by β-arrestin recruitment and continuously promoted NO synthesis. To reduce the enhanced oxidative stress, NAPDH production through pentose-phosphate pathway and resulting consumption of glucose were promoted in ALESIA-treated cells. ALESIA therefore accelerated glucose starvation of cancer cells and preferentially killed them both in vitro and in vivo.
 
Overall design We analyzed polyA-tailed RNA profiles including transcribing RNAs in MCF-7 cells of ALESIA racemate treatment (n=3) and control (n=3) under normal culture condition using Ion-proton.
Web link https://www.sciencedirect.com/science/article/pii/S2451945621000040
 
Contributor(s) Toyomoto M, Denawa M, Iida K, Hagiwara M
Citation(s) 33561428
Submission date Mar 02, 2018
Last update date Feb 12, 2021
Contact name Kei IIDA
E-mail(s) kiida@life.kindai.ac.jp
Organization name Kindai University
Street address Kowakae 3-4-1
City Higashi-Osaka
State/province Osaka
ZIP/Postal code 5778502
Country Japan
 
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (6)
GSM3028968 MCF7_ALESIA.rep1
GSM3028969 MCF7_ALESIA.rep2
GSM3028970 MCF7_ALESIA.rep3
This SubSeries is part of SuperSeries:
GSE143670 S1PR3–G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation
Relations
BioProject PRJNA436703
SRA SRP133829

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111352_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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