|
| Status |
Public on Sep 01, 2018 |
| Title |
GATA2 mutant transduction of murine hematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GATA2 mutant transduction of murine hematopoietic stem cells
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| |
|
| Overall design |
C57BL/6 8-week female were treated with 5-fluorouracil (5-FU) at 150mg/kg 6 days prior to bone marrow cell harvesting. The bone marrow cells were infected with MIE, WT and R396Q retroviral particles. GFP-positive cells were sorted after 72h of culture. Total RNA was extracted using Rneasy plus MiniKit (Qiagen) and its quality was assessed with the RNA Screen Tape on Agilent 2200 Tapestation Bioanalyzer (Agilent Technologies). RNA samples were purified and prepared according to the manufacturer's protocol using the Affymetrix's GeneChip Whole Transcript Sense Target Labeling Assay Kit (Affymetrix) and hybridized on Affymetrix GeneChip Mouse Exon 1.0 ST arrays. Probe-signal intensities were normalized and summarized using the robust multiarray average (RMA) method with the Affymetrix’s Expression Console software.
3 controls (MIE), 3 GATA2 wild type, 3 GATA2 R396Q
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| |
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| Contributor(s) |
Delabesse E, Prade N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 02, 2018 |
| Last update date |
Sep 02, 2018 |
| Contact name |
Eric DELABESSE |
| E-mail(s) |
delabesse.e@chu-toulouse.fr
|
| Organization name |
Institut Universitaire de Cancérologie de Toulouse, site Oncopôle CHU de Toulouse
|
| Street address |
1 avenue Irène Joliot-Curie
|
| City |
Toulouse |
| ZIP/Postal code |
31059 |
| Country |
France |
| |
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| Platforms (1) |
| GPL20258 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CDF version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA436636 |
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