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| Status |
Public on Mar 02, 2018 |
| Title |
RNA-seq data from NSCs and tumorspheres generated using the RCAS/TVA-CRISPR/Cas9 system |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It has been gradually established that the vast majority of human tumors are extraordinarily heterogeneous at a genetic level. To accurately recapitulate this complexity, it is now evident that in vivo animal models of cancers will require to recreate not just a handful of simple genetic alterations, but possibly dozens and increasingly intricate. Here, we have combined the RCAS/TVA system with the CRISPR/Cas9 genome editing tools for precise modelling of human tumors. We show that somatic deletion in neural stem cells (NSCs) of a variety of known tumor suppressor genes (Trp53, Cdkn2a and Pten), in combination with the expression of an oncogene driver, leads to high-grade glioma formation. Moreover, by simultaneous delivery of pairs of guide RNAs (gRNAs) we generated different gene fusions, either by chromosomal deletion (Bcan-Ntrk1) or by chromosomal translocation (Myb-Qk), and we show that they have transforming potential in vivo and in vitro.
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| Overall design |
Bcan-Ntrk1 tumorspheres, Myb-Qk gRNA transduced NSCs, Ctrl gRNA transduced NSCs, Cdkn2a gRNA PDGFB tumorspheres, Pten gRNA PDGFB tumorspheres, and Trp53 gRNA PDGFB tumorspheres. 2 replicates each.
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| Contributor(s) |
Squatrito M, Graña O |
| Citation(s) |
29654229 |
| Submission date |
Feb 15, 2018 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Osvaldo Graña |
| Organization name |
CNIO
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| Department |
Structural Biology
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| Lab |
Bioinformatics Unit
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| Street address |
C/ Melchor Fernández Almagro, 3
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| City |
Madrid |
| State/province |
Comunidad de Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA434303 |
| SRA |
SRP132952 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110700_FPKM_table.txt.gz |
811.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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