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Series GSE110700 Query DataSets for GSE110700
Status Public on Mar 02, 2018
Title RNA-seq data from NSCs and tumorspheres generated using the RCAS/TVA-CRISPR/Cas9 system
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary It has been gradually established that the vast majority of human tumors are extraordinarily heterogeneous at a genetic level. To accurately recapitulate this complexity, it is now evident that in vivo animal models of cancers will require to recreate not just a handful of simple genetic alterations, but possibly dozens and increasingly intricate. Here, we have combined the RCAS/TVA system with the CRISPR/Cas9 genome editing tools for precise modelling of human tumors. We show that somatic deletion in neural stem cells (NSCs) of a variety of known tumor suppressor genes (Trp53, Cdkn2a and Pten), in combination with the expression of an oncogene driver, leads to high-grade glioma formation. Moreover, by simultaneous delivery of pairs of guide RNAs (gRNAs) we generated different gene fusions, either by chromosomal deletion (Bcan-Ntrk1) or by chromosomal translocation (Myb-Qk), and we show that they have transforming potential in vivo and in vitro.
Overall design Bcan-Ntrk1 tumorspheres, Myb-Qk gRNA transduced NSCs, Ctrl gRNA transduced NSCs, Cdkn2a gRNA PDGFB tumorspheres, Pten gRNA PDGFB tumorspheres, and Trp53 gRNA PDGFB tumorspheres. 2 replicates each.
Contributor(s) Squatrito M, Graña O
Citation(s) 29654229
Submission date Feb 15, 2018
Last update date Mar 08, 2019
Contact name Osvaldo Graña
Organization name CNIO
Department Structural Biology
Lab Bioinformatics Unit
Street address C/ Melchor Fernández Almagro, 3
City Madrid
State/province Comunidad de Madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM3014717 Bcan_Ntrk_1
GSM3014718 Bcan_Ntrk_2
GSM3014719 Myb_Qk_1
BioProject PRJNA434303
SRA SRP132952

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Supplementary file Size Download File type/resource
GSE110700_FPKM_table.txt.gz 811.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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