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Series GSE110649 Query DataSets for GSE110649
Status Public on Sep 18, 2019
Title RNA sequencing of wildtype-KRAS HKE3 (wtHKE3) and mutant-KRAS HKE3 (mtHKE3) colorectal cancer cell lines before and after stimulation with TGF-alpha
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing was performed on wtHKE3 and mtHKE3 colorectal cancer cell lines (obtained from Shirasawa [1]) before stimulation (timepoint 0) and at 15, 30, 60, 90 and 120 mins post-stimulation with transforming growth factor alpha (TGF-α) (0.01 µg/mL, Abcam). Three replicates were sequenced at each timepoint. [1] Tsunoda, Ishikura, Doi et al., Anticancer Res 2015, 35(8):4453-4459.
 
Overall design RNAseq profiles from wtHKE3 and mtHKE3 cells before and after stimulation with TGF-alpha. In total, 36 cDNA libraries (2 cell lines X 6 time-points X 3 replicates per timepoint) were generated for sequencing.
 
Contributor(s) Charitou T, Srihari S, Lynn DJ
Citation(s) 31133691
Submission date Feb 15, 2018
Last update date Sep 18, 2019
Contact name Sriganesh Srihari
E-mail(s) sriganesh.srihari@sahmri.com
Phone +61881284066
Organization name South Australian Health and Medical Research Institute
Department Infection and Immunity
Lab EMBL-Australia Lynn Group
Street address North Terrace
City Adelaide
State/province SA
ZIP/Postal code 5007
Country Australia
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (36)
GSM3004821 Unstimulated wtHKE3 cells (time 0 mins) - Replicate 1
GSM3004822 Unstimulated wtHKE3 cells (time 0 mins) - Replicate 2
GSM3004823 Unstimulated wtHKE3 cells (time 0 mins) - Replicate 3
Relations
BioProject PRJNA434191
SRA SRP132910

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110649_RNAseq_Counts_wtHKE3_mtHKE3.txt.gz 1.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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