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Series GSE110629 Query DataSets for GSE110629
Status Public on Mar 01, 2018
Title Next-generation sequencing of three memory OT-I cell subsets from mice infected with Listeria monocytogenes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: RNA-seq analysis of three memory OT-I cell subsets (from a Klrg1-Cre fate reporter mouse model) isolated from the spleen of C57BL/6 mice infected with Listeria monocytogenes. The hypothesis tested in the present study was that KLRG1+ effector CD8 T lymphocytes differentiate into KLRG1- memory CD8 T lymphocytes and provide long-lasting immunity against infectious diseases and malignancies.
Methods: Total RNA was obtained from FACS-purified OT-I cell subsets isolated from spleen 104 (experiment 1) and 110 days post infection (experiment 2) with ovalbumin-expressing Listeria monocytogenes (LM-OVA).
Results: Using RNA-seq technology, we performed genome-wide transcriptional profiling of three memory OT-I cells (KLRG1+ Reporter+, KLRG1- Reporter+ (exKLRG1) and KLRG1- Reporter-) and identified 36 genes differentially expressed (> 1.5-fold) between exKLRG1 and KLRG1- Reporter- memory OT-I cells, and 132 differentially expressed genes between exKLRG1 and KLRG1+ Reporter+ memory OT-I cells. We then confirmed the expression of 15 genes/molecules by qRT-PCR and/or flow cytometry.
Conclusions: Our study represents the first fate mapping analysis of KLRG1+ effector OT-I cells, demonstrates that KLRG1+ effector OT-I cells differentiate into all memory T cell lineages thereby promoting protective immunity. RNA-seq also identified CX3CR1 as a marker of circulating exKLRG1 early memory OT-I cells.
 
Overall design Total RNA was obtained from FACS-purified OT-I cell subsets isolated from spleen 104-110 days post infection with ovalbumin-expressing Listeria monocytogenes (LM-OVA).
 
Contributor(s) Ishigame H
Citation(s) 29625895
Submission date Feb 14, 2018
Last update date Sep 12, 2018
Contact name Dietmar Herndler-Brandstetter
Organization name Medical University of Vienna
Department Institute of Cancer Research
Street address Borschkegasse 8a
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (6)
GSM3004524 KLRG1- Reporter- OT-I cells (exp1)
GSM3004525 KLRG1+ Reporter+ OT-I cells (exp1)
GSM3004526 KLRG1- Reporter+ OT-I cells (exp1)
This SubSeries is part of SuperSeries:
GSE110707 KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate Into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity
Relations
BioProject PRJNA434131
SRA SRP132888

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Supplementary file Size Download File type/resource
GSE110629_Matrix_table.txt.gz 442.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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