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Series GSE110501 Query DataSets for GSE110501
Status Public on Feb 28, 2018
Title Single-cell transcriptome analyses reveal endothelial cell heterogeneity in tumors and changes following anti-angiogenic treatment
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To understand tumor stromal cell heterogeneity focusing on tumor endothelial cells and tumor-associated fibroblasts, we isolated single cells from COLO205 tumor xenograft grown sub-cutaneously in SCID mice. To enrich stromal cell content, we removed vast majority of tumor cells by a depletion protocol using anti-CD24 and anti-E-Cadherin antibodies. The remaining single cells were profiled by single cell RNAseq
Overall design Tumors were dissociated, sorted into single cells, and profiled by 1 chromium. Single cell 3' library, Gelbeads and multiplex kit were made by using 10x Genomics platform.
Contributor(s) Thurston G
Citation(s) 29449267
Submission date Feb 12, 2018
Last update date Mar 25, 2019
Contact name Qi Zhao
Organization name Regeneron Pharmaceuticals Inc
Street address 777 Old Saw Mill River Road
City Tarrytown
ZIP/Postal code 10591
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
Samples (16)
GSM2994872 COLO205.xenograft.hFc.24hr.mm10
GSM2994873 COLO205.xenograft.aflibercept.24hr.mm10
GSM2994874 COLO205.xenograft.anti-Dll4-Ab.24hr.mm10
BioProject PRJNA433865
SRA SRP132721

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110501_RAW.tar 48.9 Mb (http)(custom) TAR (of TXT)
GSE110501_genes.gtf.gz 5.8 Mb (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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