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Series GSE110374 Query DataSets for GSE110374
Status Public on Apr 25, 2018
Title Ribotag analysis of proprioceptive dorsal root ganglion neurons in cell culture
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We analyzed ribosome-associated RNA (raRNA) in dorsal root ganglion neurons in TrkC+ proprioceptive dorsal root ganglion neurons in cell culture.
 
Overall design All DRG neurons from young adult (3-6 mo) male mice expressing Rpl22HA/HA Runx3-Cre+ and age/sex matched Cre negative littermates (Rpl22HA/HA Runx3-Cre-) were harvested using density purification on Percoll and grown for 24h in serum-supplemented F12 medium. Ribosome-associated RNA was isolated using immunoprecipitation targeting the HA epitope. 10% of pre-immunoprecipitation lysates were used for input samples reflecting total RNA profile of the tissue. Cre negative samples were sequenced to control for background. N=3 for all four groups (Pulldown/Cre+, Pulldown/Cre-, Input/Cre+, Input/Cre-).
 
Contributor(s) Kawaguchi R
Citation(s) 29756027
Submission date Feb 08, 2018
Last update date Jun 26, 2019
Contact name Riki Kawaguchi
E-mail(s) rkawaguchi@mednet.ucla.edu
Phone 4244424783
Organization name University of California Los Angeles
Department Department of Neurology and Department of Psychiatry
Lab Informatics Center for Neurogenetics and Neurogenomics (ICNN).
Street address 760 Westwood Plaza, Room 37-420
City Los Angeles
State/province CA
ZIP/Postal code 90095-1759
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (12)
GSM2989239 Runx3_PD_1
GSM2989240 Runx3_PD_2
GSM2989241 Runx3_PD_3
Relations
BioProject PRJNA433551
SRA SRP132512

Download family Format
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Supplementary file Size Download File type/resource
GSE110374_Complete_genelist_CrePD_vs_input.csv.gz 1.4 Mb (ftp)(http) CSV
GSE110374_Complete_genelist_wtPD_vs_input.csv.gz 2.2 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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