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Series GSE110227 Query DataSets for GSE110227
Status Public on Feb 04, 2019
Title DNA methylation change involved in doxorubicin-induced testicular toxicity in mice
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Testicular toxicity is one of the frequent adverse effects of cancer chemotherapy and the problem is that there is no effective biomarker. To find effective biomarkers, we focused on epigenetic mechanisms of male germline. Therefore, our study investigated the DNA methylation status of male germline under the testicular toxicity induced by doxorubicin(DXR), a widely used anticancer agent. We established mouse models of initial stage of testicular toxicity and testicular pre-toxicity by administrating 0.2 mg/kg and 0.02 mg/kg of DXR twice a week for 5 weeks. Western blotting analysis revealed the protein expression levels of DNA methyltransferases DNMT3a and DNMT3b were decreased in both the DXR administration groups. Consistently, comprehensive DNA methylation analysis of sperm DNA using MBD-seq revealed that the majority of methylation changes induced by DXR administration were hypomethylation. This study showed the possibility of early diagnosis of testicular toxicity by examining DNA methylation status of sperm.
Overall design DNA methylation profiles of sperms obtained from Control and doxorubicin(DXR) treated mice were analyzed using MBD2 mediated methylated DNA enrichment followed by next generation sequencing (MBD-seq) with Illumina MiSeq.
Contributor(s) Tanemura K, Sakai K, Otsuka M, Igarashi K
Citation(s) 29524425
Submission date Feb 06, 2018
Last update date May 21, 2019
Phone +81-3-3786-1011
Organization name Hoshi University School of Pharmacy and Pharmaceutical Science
Department Life Science Tokyo Advanced Research center (L-StaR)
Street address 2-4-41 Ebara
City Shinagawa-ku
State/province Tokyo
ZIP/Postal code 142-8501
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (3)
GSM2982986 Vehicle control
GSM2982987 Low dose (0.02mg/kg) DXR treated
GSM2982988 High dose (0.2mg/kg) DXR treated
BioProject PRJNA433228
SRA SRP132306

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Supplementary file Size Download File type/resource
GSE110227_DXR_Sperm_DNAmethylation_RMS_w_genic_annotation.xlsx 36.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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