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Series GSE11011 Query DataSets for GSE11011
Status Public on Apr 01, 2008
Title eIF4GI Links Nutrient Sensing by mTOR to Cell Proliferation and Inhibition of Autophagy
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Translation initiation factors have complex functions in cells which are not yet understood. We show that depletion of initiation factor eIF4GI only modestly reduces overall protein synthesis in cells, but phenocopies nutrient-starvation or inhibition of protein kinase mTOR, a key nutrient sensor. eIF4GI depletion impairs cell proliferation, bioenergetics and mitochondrial activity, thereby promoting autophagy. Translation of mRNAs involved in cell growth, proliferation and bioenergetics were selectively inhibited by reduction of eIF4GI, whereas mRNAs encoding proliferation inhibitors and catabolic pathway factors were increased. Depletion or over-expression of other eIF4G family members did not recapitulate these results. The majority of mRNAs that were translationally impaired with eIF4GI depletion were excluded from polyribosomes due to the presence of multiple upstream open reading frames and low mRNA abundance. These results suggest that the high levels of eIF4GI observed in many breast cancers might act to specifically increase proliferation, prevent autophagy and release tumor cells from control by nutrient sensing.
Global regulation of transcription and polysomal association in eIF4GI-silenced cells.
Keywords: Gene Silencing
 
Overall design Two sets of experiments are presented. First, overall transcriptome changes were determined for control or eIF4GI silenced cells. Next, mRNAs associated with polysomes were compared between control and eIF4GI silenced cells.
 
Contributor(s) Ramirez-Valle F, Braunstein S, Zavadil J, Fermenti SC, Schneider RJ
Citation(s) 18426977
Submission date Apr 01, 2008
Last update date Dec 06, 2018
Contact name Robert J Schneider
E-mail(s) robert.schneider@nyumc.org
Phone +1-212-263-6006
Organization name NYU School of Medicine
Department Microbiology
Street address 550 First Ave, MSB 238
City New York
State/province NY
ZIP/Postal code 10016
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM278478 human_MCF10A_siRNA-eIF4GI_polysomal RNA_R1
GSM278479 human_MCF10A_siRNA-eIF4GI_polysomal RNA_R2
GSM278480 human_MCF10A_siRNA-eIF4GI_polysomal RNA_R3
Relations
BioProject PRJNA107097

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11011_RAW.tar 21.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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