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Status |
Public on Dec 05, 2019 |
Title |
Single cell RNA sequencing time-course of mesenchymal progenitors during regeneration after notexin induced muscle injury |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
After tissue injury mesenchymal progenitors undergo a transient proliferative expansion, morphological transition and migration from their perivascular niche. To gain transcriptomic insights into the MP function in regeneration, a muscle-injury model was employed to activate MPs and follow their contribution to tissue regeneration. To compliment the population RNA seq profile previously obtained and to uncover the suspected heterogeneity of MPs in this context, tdTomato positive cells from selected timepoints were profiled by single cell RNA sequencing.
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Overall design |
Single cell RNA-seq analyes of enriched mesenchymal progenitors at selected timepoints during skeletal muscle regeneration.
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Contributor(s) |
Scott RW, Underhill TM |
Citation(s) |
31809738 |
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Submission date |
Feb 01, 2018 |
Last update date |
Feb 19, 2020 |
Contact name |
Tully Michael Underhill |
E-mail(s) |
tunderhi@brc.ubc.ca
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Phone |
604-822-5833
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Organization name |
University of British Columbia
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Department |
Biomedical Research Centre
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Street address |
2222 Health Sciences Mall
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City |
Vancouver |
State/province |
British Columbia |
ZIP/Postal code |
V6T 1Z3 |
Country |
Canada |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (5)
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GSM2976778 |
Single cell RNA-seq tdTomato MPs quiescent |
GSM2976779 |
Single cell RNA-seq tdTomato MPs 1 day post notexin injury |
GSM2976780 |
Single cell RNA-seq tdTomato MPs 2 day post notexin injury |
GSM2976781 |
Single cell RNA-seq tdTomato MPs 4 day post notexin injury |
GSM2976782 |
Single cell RNA-seq tdTomato MPs 14 day post notexin injury |
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This SubSeries is part of SuperSeries: |
GSE110038 |
Hic1 defines quiescent mesenchymal progenitor subpopulations with distinct functions and fates in skeletal muscle regeneration |
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Relations |
BioProject |
PRJNA432604 |