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| Status |
Public on Feb 06, 2019 |
| Title |
Neutrophils Escort Circulating Tumor Cells to Enable Cell Cycle Progression |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Circulating tumor cells (CTCs) are precursors of metastasis in several cancer types, and are occasionally found within the bloodstream in association with non-malignant cells such as white blood cells (WBCs). The identity and function of these CTC-associated WBCs, as well as the molecular features that define the interaction between WBCs and CTCs are unknown. Here, we achieve the isolation and interrogation of individual CTC-associated WBCs, alongside with corresponding cancer cells within each CTC-WBC-cluster, from multiple breast cancer patients and mouse models.
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| Overall design |
Single-cell RNA-Seq from CTCs and CTC-WBC-clusters obtained from multiple breast cancer patients and mouse models were used to identify CTC-associated WBCs cell types and differential expression between CTCs alone and CTC-associated to WBC.
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| Contributor(s) |
Szczerba B, Castro-Giner F, Vetter M, Gkountela S, Landin J, Krol I, Donato C, Scherrer R, Singer J, Beisel C, Kurzeder C, Weber WP, Rochlitz C, Heinzelmann-Schwarz V, Beerenwinkel N, Aceto N |
| Citation(s) |
30728496, 32905777 |
| Submission date |
Jan 29, 2018 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Francesc Castro-Giner |
| Organization name |
ETH Zurich
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| Street address |
Otto-​Stern-Weg 7
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| City |
Zurich |
| ZIP/Postal code |
CH-8093 |
| Country |
Switzerland |
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| Platforms (3) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
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| Samples (470)
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| Relations |
| BioProject |
PRJNA431985 |
| SRA |
SRP131647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109761_RAW.tar |
62.9 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE109761_processed_normalized_matrix_hs.txt.gz |
25.8 Mb |
(ftp)(http) |
TXT |
| GSE109761_processed_normalized_matrix_mm.txt.gz |
8.2 Mb |
(ftp)(http) |
TXT |
| GSE109761_sce_hs.rds.gz |
42.4 Mb |
(ftp)(http) |
RDS |
| GSE109761_sce_mm.rds.gz |
14.9 Mb |
(ftp)(http) |
RDS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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