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Status |
Public on Jul 11, 2019 |
Title |
Profiling chromatin state by single-cell itChIP-seq [itChIP-seq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We present a low-cost, generalizable ChIP-seq (itChIP), compatible to both low-input and single cells for profiling chromatin states. This method combines chromatin opening, simultaneous cellular indexing and chromatin tagmentation in a single tube. Single-cell itChIP data yield ~ 5000 unique reads per cell, sufficiently defining cell identifies and subpopulations of a given cell type. Our results demonstrate that itChIP is a generalizable technology for single-cell chromatin profiling of samples limited to ultra-low number of cells.
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Overall design |
Based on Tn5 transposition of chromatin, the generalizable itChIP combines chromatin opening, simultaneous cellular indexing and chromatin tagmentation in a single tube.
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Contributor(s) |
Ai S, Xiong H, Li C, Luo Y, Shi Q, He A |
Citation(s) |
31481796 |
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Submission date |
Jan 29, 2018 |
Last update date |
Oct 10, 2019 |
Contact name |
Chen Li |
E-mail(s) |
chenli_imm@pku.edu.cn
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Organization name |
Peking University
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Department |
Institute of Molecular Medicine
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Lab |
Aibin He Lab
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Street address |
Yihe Yuan Road No.5
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City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100871 |
Country |
China |
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Platforms (3) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
GPL21273 |
HiSeq X Ten (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (43)
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This SubSeries is part of SuperSeries: |
GSE109762 |
Profiling chromatin state by single-cell itChIP-seq |
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Relations |
BioProject |
PRJNA431987 |
SRA |
SRP131644 |