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Series GSE109732 Query DataSets for GSE109732
Status Public on Mar 29, 2018
Title Development of plasma cell-prone germinal center B cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Higher- or lower-affinity GC B cells are directed to plasma cell or recycling GC cell fates; however, how commitment to the plasma cell fate takes place is unclear. By using the level of Bcl6 as a marker, we found that a population of light zone (LZ) GC cells, Bcl6loCD69hi with IRF4 and higher-affinity BCRs or Bcl6hiCD69hi with lower- affinity BCRs, favored the plasma cell or recycling GC cell fate, respectively. Mechanistically, CD40 acted as a dose-dependent regulator for Bcl6loCD69hi cell formation. Furthermore, we found that ICAM1 and SLAM levels on Bcl6loCD69hi cells were higher than on Bcl6hiCD69hi cells, thereby affording more stable TFH-GC B cell contacts, while attenuating this contact down-regulated IRF4. These data support the model that commitment to the plasma cell begins in the GC, and suggest that stability and possibly duration of TFH-GC B cell contacts are key for formation of plasma cell-prone precursor GC cells.
Overall design mRNA profiles of GC B cell subsets and GC-derived plasmablast were generated by barcode-based digital RNA sequencing.
Contributor(s) Ise W, Shiroguchi K, Kurosaki T
Citation(s) 29669250
Submission date Jan 26, 2018
Last update date Jan 27, 2019
Contact name Eri Nishiyama
Organization name Riken
Department Center for Biosystems Dynamics Research
Lab Laboratory for Prediction of Cell Systems Dynamics
Street address 6-2-3 Furuedai
City Suita
State/province Osaka
ZIP/Postal code 565-0874
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (15)
GSM2957687 DZ 1
GSM2957688 DZ 2
GSM2957689 DZ 3
BioProject PRJNA431808
SRA SRP131526

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Supplementary file Size Download File type/resource
GSE109732_GeneExpression_DZ_Fraction1-3_GC-PB.txt.gz 515.1 Kb (ftp)(http) TXT
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