|
| Status |
Public on Jun 04, 2018 |
| Title |
Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single progenitors and demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed that EMPs express a unique hybrid basal and luminal signature and the factors associated with the different lineages. Sustained p63 expression in EMPs promotes unipotent BC fate and was sufficient to reprogram adult LCs into BCs by promoting an intermediate hybrid multipotent like state. Altogether, this study identifies the timing and the mechanisms mediating the early lineage segregation of multipotent progenitors during MG development.
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| |
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| Overall design |
RNAsequencing of mouse mammary gland cells
|
| |
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| Contributor(s) |
Blanpain C, Brisebarre A, Van Keymeulen A |
| Citation(s) |
29784918 |
| |
| Submission date |
Jan 26, 2018 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Alexandra Van Keymeulen |
| Organization name |
Université Libre de Bruxelles
|
| Department |
IRIBHM
|
| Lab |
Blanpain
|
| Street address |
808, route de Lennik CP602
|
| City |
Bruxelles |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE109711 |
Early lineage segregation of multipotent embryonic mammary gland progenitors |
|
| Relations |
| BioProject |
PRJNA431773 |
| SRA |
SRP131487 |
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