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Series GSE109707 Query DataSets for GSE109707
Status Public on Jun 04, 2018
Title Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single progenitors and demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed that EMPs express a unique hybrid basal and luminal signature and the factors associated with the different lineages. Sustained p63 expression in EMPs promotes unipotent BC fate and was sufficient to reprogram adult LCs into BCs by promoting an intermediate hybrid multipotent like state. Altogether, this study identifies the timing and the mechanisms mediating the early lineage segregation of multipotent progenitors during MG development.
Overall design RNAsequencing of mouse mammary gland cells
Contributor(s) Blanpain C, Brisebarre A, Van Keymeulen A
Citation(s) 29784918
Submission date Jan 26, 2018
Last update date Mar 08, 2019
Contact name Alexandra Van Keymeulen
Organization name Université Libre de Bruxelles
Department IRIBHM
Lab Blanpain
Street address 808, route de Lennik CP602
City Bruxelles
ZIP/Postal code 1070
Country Belgium
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM2948737 AW1-CD1-LC1.1
GSM2948738 AW2-CD1-LC2.2
GSM2948739 AW3-K8P63-LC1.1
This SubSeries is part of SuperSeries:
GSE109711 Early lineage segregation of multipotent embryonic mammary gland progenitors
BioProject PRJNA431773
SRA SRP131487

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Supplementary file Size Download File type/resource
GSE109707_CP20M-Wuidart.txt.gz 1.4 Mb (ftp)(http) TXT
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