GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE109645 Query DataSets for GSE109645
Status Public on Nov 06, 2018
Title Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Developing an effective xeno-free and defined system for human keratinocyte culture has been one of the fundamental measures in current cellular therapy. For keratinocytes, in particular, such system will not only fulfill clinical safety and quality criteria, it will allow for the management of less severe burns and other skin injuries such as chronic wounds. To date, the expansion of autologous keratinocytes in the clinical settings still relies on 3T3 feeder co-culture system. Here we report a completely xeno-free and defined culture system by using human recombinant laminins as feeder replacement. We have thoroughly characterized the cells cultured in this new system both in vitro and in vivo. We found that laminin-511/-521, and the unanticipated laminin-411/-421, but not -332, -111, -121, -211, or LN-221 support adult human skin keratinocytes and could maintain their self-renewal properties comparable to the 3T3 system. We believe our new culture method should facilitate broader use of cultured epithelial cell products for today’s regenerative medicine.
Overall design RNA sequencing and analysis on whole adult human skin or freshly cultured human keratinocytes using two different methods: co-cultured with 3T3 feeder and laminin-coated tissue culture plates.
Contributor(s) Tjin MS, Chua AW, Moreno-Moral A, Buenaflor BE, Chong LY, Petretto E, Tan BK, Tryggvason K
Citation(s) 30377295
Submission date Jan 25, 2018
Last update date Dec 23, 2018
Contact name Aida Moreno-Moral
Organization name Duke-NUS Medical School
Street address 8 College Road
City Singapore
ZIP/Postal code 169857
Country Singapore
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (18)
GSM2947809 15006_3T3
GSM2947810 15006_421
GSM2947811 15006_511
BioProject PRJNA431596
SRA SRP131198

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109645_TPM_keratinocytesLN.txt.gz 956.0 Kb (ftp)(http) TXT
GSE109645_gene_counts_keratinocytesLN.txt.gz 910.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap