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Series GSE109591 Query DataSets for GSE109591
Status Public on Jan 01, 2019
Title The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Human p300 is a transcriptional co-activator and a major acetyltransferase that acetylates histones and other proteins facilitating gene transcription. The activity of p300 relies on the fine-tuned interactome that involves a dozen p300 domains and hundreds of binding partners and links p300 to a wide range of vital signaling events. Here, we report on a novel function of the ZZ-type zinc finger (ZZ) of p300 as a reader of histone H3. We show that the ZZ domain and acetyllysine recognizing bromodomain (BD) of p300 play critical roles in modulating p300 enzymatic activity and its association with chromatin. Acetyllysine binding of BD is essential for acetylation of histones H3 and H4, whereas interaction of the ZZ domain with H3 promotes selective acetylation of histone H3K27 and H3K18.
Overall design ChIP-seq of Flag-p300, H3K27ac, H3K18ac were applied in human H1299 cells stably expressing WT or mutant (N1671A/D1690A) p300-BRPHZT fragment and control vector (V).
Contributor(s) Shi J, Xue Y, Shi X
Citation(s) 30150647
Submission date Jan 24, 2018
Last update date May 02, 2019
Contact name Jiejun Shi
Organization name University of California Irvine
Department School of Medicine
Lab Li lab
Street address 5270 California Ave
City Irvine
State/province CA
ZIP/Postal code 92617
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (13)
GSM2946880 Flag-p300 ChIP-seq in N1671A
GSM2946881 H3K18ac ChIP-seq in N1671A
GSM2946882 H3K27ac ChIP-seq in N1671A
BioProject PRJNA431434
SRA SRP131273

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Supplementary file Size Download File type/resource
GSE109591_RAW.tar 5.3 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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