|
Status |
Public on Jun 06, 2018 |
Title |
HDAC genes play distinct and redundant roles in Cryptococcus neoformans virulence |
Organism |
Cryptococcus neoformans var. grubii H99 |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The human fungal pathogen Cryptococcus neoformans undergoes many phenotypic changes to promote its survival in specific ecological niches and inside the host. To explore the role of chromatin remodeling on the expression of virulence-related traits, we identified and deleted seven genes encoding predicted class I/II histone deacetylases (HDACs) in the C. neoformans genome. Our results identified the HDA1 HDAC gene as a central mediator controlling several cellular processes, including mating and virulence. A global gene expression profile comparing the hda1Δ mutant versus wild-type revealed altered transcription of specific genes associated with the most prominent virulence attributes in this fungal pathogen. This study directly correlates the effects of Class I/II HDAC-mediated chromatin remodeling on the marked phenotypic plasticity and virulence potential of this microorganism. Furthermore, our results provide insights into regulatory mechanisms involved in virulence gene expression that are likely shared with other microbial pathogens.
|
|
|
Overall design |
Gene expression analysis of WT and hda1∆ mutant cells generated in triplicate by RNA-Sequencing
|
|
|
Contributor(s) |
Brandão F, Esher SK, Alspaugh JA |
Citation(s) |
29581526 |
|
Submission date |
Jan 24, 2018 |
Last update date |
Jun 06, 2018 |
Contact name |
Shannon Esher |
E-mail(s) |
shannon.esher@duke.edu
|
Phone |
(919) 684-5054
|
Organization name |
Duke University
|
Department |
Molecular Genetics and Microbiology
|
Lab |
Alspaugh
|
Street address |
337 Sands Building, 303 Research Drive
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27710 |
Country |
USA |
|
|
Platforms (1) |
GPL21548 |
Illumina HiSeq 2000 (Cryptococcus neoformans var. grubii H99) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA431436 |
SRA |
SRP131272 |