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Series GSE109582 Query DataSets for GSE109582
Status Public on Jun 06, 2018
Title HDAC genes play distinct and redundant roles in Cryptococcus neoformans virulence
Organism Cryptococcus neoformans var. grubii H99
Experiment type Expression profiling by high throughput sequencing
Summary The human fungal pathogen Cryptococcus neoformans undergoes many phenotypic changes to promote its survival in specific ecological niches and inside the host. To explore the role of chromatin remodeling on the expression of virulence-related traits, we identified and deleted seven genes encoding predicted class I/II histone deacetylases (HDACs) in the C. neoformans genome. Our results identified the HDA1 HDAC gene as a central mediator controlling several cellular processes, including mating and virulence. A global gene expression profile comparing the hda1Δ mutant versus wild-type revealed altered transcription of specific genes associated with the most prominent virulence attributes in this fungal pathogen. This study directly correlates the effects of Class I/II HDAC-mediated chromatin remodeling on the marked phenotypic plasticity and virulence potential of this microorganism. Furthermore, our results provide insights into regulatory mechanisms involved in virulence gene expression that are likely shared with other microbial pathogens.
 
Overall design Gene expression analysis of WT and hda1∆ mutant cells generated in triplicate by RNA-Sequencing
 
Contributor(s) Brandão F, Esher SK, Alspaugh JA
Citation(s) 29581526
Submission date Jan 24, 2018
Last update date Jun 06, 2018
Contact name Shannon Esher
E-mail(s) shannon.esher@duke.edu
Phone (919) 684-5054
Organization name Duke University
Department Molecular Genetics and Microbiology
Lab Alspaugh
Street address 337 Sands Building, 303 Research Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platforms (1)
GPL21548 Illumina HiSeq 2000 (Cryptococcus neoformans var. grubii H99)
Samples (6)
GSM2946796 WT-1
GSM2946797 WT-2
GSM2946798 WT-3
Relations
BioProject PRJNA431436
SRA SRP131272

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109582_DESeq_results.csv.gz 282.1 Kb (ftp)(http) CSV
GSE109582_H99_v_hda1_counts.csv.gz 118.8 Kb (ftp)(http) CSV
GSE109582_RAW.tar 8.7 Mb (http)(custom) TAR (of CXB)
GSE109582_cuffdiff_101817_gene_exp.csv.gz 358.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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