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| Status |
Public on Aug 06, 2019 |
| Title |
Transcriptome analysis of differentiated normal mouse urothelial organoids reveals novel pathways activated during urothelial differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptomic analysis revealed significant changes between organoids growing in full medium growth conditions (proliferative NMU-o) and deprived medium (differentiated NMU-o). Around 4100 genes are differentially expressed between both conditions. Moreover, GSEA analysis in proliferation showed pathways related to cell cycle, DNA repair, RNA biology and protein synthesis; in differentiation, pathways related to cellular traffic, cell-cell adhesion and notch signalling are up-regulated.
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| Overall design |
We established normal mouse urothelial organoids (NMU-o) from 3 independent samples and further induced differentiation. For each individual sample we sequenced the corresponding proliferation and differentiation stages.
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| Contributor(s) |
Santos CP, Millán I, Carrillo-de Santa Pau E, Real FX |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 24, 2018 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Enrique Carrillo de Santa Pau |
| E-mail(s) |
enrique.carrillo@imdea.org
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| Organization name |
Spanish National Cancer Research Centre (CNIO)
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| Department |
Cancer Cell Biology Programme
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| Lab |
Epithelial Carcinogenesis group
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| Street address |
C/ Melchor Fernández Almagro, 3
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| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA431400 |
| SRA |
SRP131374 |
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