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| Status |
Public on Aug 21, 2018 |
| Title |
Whole-genome mapping of RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac in human pre-B lymphoblasts, and B cell precursor leukemia (BCP-ALL) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Genome binding/occupancy profiling of human Runt-related transcription factor 1 (RUNX1), Far Upstream Binding Protein 1 (FUBP1) and histone marks by high throughput sequencing. RUNX1 and FUBP1 are two key transcriptional regulators implicated in hematopoiesis, from the maintenance of HSC to lineage-specific differentiation. We hypothesized that both proteins could play a joint role in transcription regulation of hematologic lineages. Here, we investigated the molecular proximity between RUNX1 and FUBP1 proteins on chromatin and their common target genes in pre-B lymphoblasts and B cell precursor leukemia (BCP-ALL). Altogether, our data show that RUNX1 and a subset of FUBP1 colocalize on chromatin in transcriptionally active/accessible chromatin regions mainly in promoters and enhancers regions.
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| Overall design |
RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac genome-wide distributions were determined using ChIP-seq. Cells used in this study are human pre-B lymphoblast Nalm6 cell line and blasts from B-cell precursor acute lymphoblastic leukemia (BCP-ALL). ChIP samples and libraries were done by Sérandour A.A and Debaize L.
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| Contributor(s) |
Troadec M, Debaize L |
| Citation(s) |
30500954 |
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| Submission date |
Jan 18, 2018 |
| Last update date |
Jun 19, 2020 |
| Contact name |
Gilles Salbert |
| Organization name |
University Rennes 1
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| Department |
IGDR UMR6290
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| Street address |
263 Av. Gal. Leclerc
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| City |
Rennes |
| ZIP/Postal code |
35200 |
| Country |
France |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA430712 |
| SRA |
SRP131053 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109377_RAW.tar |
5.5 Gb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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