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Series GSE109377 Query DataSets for GSE109377
Status Public on Aug 21, 2018
Title Whole-genome mapping of RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac in human pre-B lymphoblasts, and B cell precursor leukemia (BCP-ALL)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genome binding/occupancy profiling of human Runt-related transcription factor 1 (RUNX1), Far Upstream Binding Protein 1 (FUBP1) and histone marks by high throughput sequencing. RUNX1 and FUBP1 are two key transcriptional regulators implicated in hematopoiesis, from the maintenance of HSC to lineage-specific differentiation. We hypothesized that both proteins could play a joint role in transcription regulation of hematologic lineages. Here, we investigated the molecular proximity between RUNX1 and FUBP1 proteins on chromatin and their common target genes in pre-B lymphoblasts and B cell precursor leukemia (BCP-ALL). Altogether, our data show that RUNX1 and a subset of FUBP1 colocalize on chromatin in transcriptionally active/accessible chromatin regions mainly in promoters and enhancers regions.
Overall design RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac genome-wide distributions were determined using ChIP-seq. Cells used in this study are human pre-B lymphoblast Nalm6 cell line and blasts from B-cell precursor acute lymphoblastic leukemia (BCP-ALL). ChIP samples and libraries were done by Sérandour A.A and Debaize L.
Contributor(s) Troadec M, Debaize L
Citation(s) 30500954
Submission date Jan 18, 2018
Last update date Jun 19, 2020
Contact name Gilles Salbert
Organization name University Rennes 1
Department IGDR UMR6290
Street address 263 Av. Gal. Leclerc
City Rennes
ZIP/Postal code 35200
Country France
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (13)
GSM2940701 Nalm6_Runx1_rep1
GSM2940702 Nalm6_Runx1_rep2
GSM2940705 Nalm6_input_rep1
BioProject PRJNA430712
SRA SRP131053

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Supplementary file Size Download File type/resource
GSE109377_RAW.tar 5.5 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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