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Series GSE109220 Query DataSets for GSE109220
Status Public on Jan 17, 2018
Title miRNA expression data from human PBMC derived form acute infectious mononucleosis patients
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Epstein Barr virus (EBV) is the etiological agent of acute infectious mononucleosis (IM). Since acute IM is a self-resolving disease with most patients regaining health in one to three weeks there have been few studies examining molecular signatures in early acute stages of the disease. microRNAs have been shown, however, to influence immune cell function and consequently the generation of antibody responses in IM. In this study, we performed a comprehensive analysis of differentially expressed microRNAs in early stage uncomplicated acute IM.
We used microarrays to profile microRNAs from patient peripheral blood obtained at the time of IM diagnosis and at subsequent time points, and identified differentially regulated microRNAs in this process.
Overall design Peripheral blood samples were derived from symptomatic patients at diagnosis, 1 , 2 and 7 months and processed to obtain PBMCs which were frozen and then maintained in liquid nitrogen until use. PBMCs were also isolated from blood derived from healthy age-matched individuals. Patient (n = 16) and healthy control (n = 3) PBMCs were suspended and lysed in TRIzol Reagent and cleaned-up with RNeasy spin columns using a modified protocol for isolation of total RNA and hybridized on Affymetrix GeneChip miRNA 4.0 arrays.
Contributor(s) Kaul V, Krams S, Martinez OM
Citation(s) 29379474
NIH grant(s)
Grant ID Grant title Affiliation Name
U01 AI104342 Biomarkers for Post-Transplant Lymphoproliferative Disorders in Children STANFORD UNIVERSITY CARLOS O ESQUIVEL
R21 AI119686 Plasmacytoid Dendritic Cell microRNAS in Transplantation STANFORD UNIVERSITY Sheri M. Krams
R21 AI115313 Targeting B Cell MicroRNA in Post-Transplant EBV-Associated B Cell Lymphoma STANFORD UNIVERSITY Olivia M. Martinez
Submission date Jan 16, 2018
Last update date Apr 18, 2018
Contact name Vandana Kaul
Organization name Stanford University
Department Surgery
Lab Transplant Immunology Lab (TIL)
Street address 1201 Welch Rd., MSLS P328
City Stanford
State/province CA
ZIP/Postal code 94305-5731
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (19)
GSM2935530 PBMC_IMDiagnosis_Subject1
GSM2935531 PBMC_IMDiagnosis_Subject2
GSM2935532 PBMC_IMDiagnosis_Subject3
BioProject PRJNA430181

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Supplementary file Size Download File type/resource
GSE109220_RAW.tar 13.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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