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Series GSE109133 Query DataSets for GSE109133
Status Public on Jan 16, 2018
Title RNA bisulfite sequencing of candidate tRNAs in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Methylation profiling by high throughput sequencing
Summary Dnmt2 and NSun2 are (cytosine-5) RNA methyltransferases. Using CRISPR/Cas9 we created null mutations in Dnmt2 and NSun2 genes in Drosophila melanogaster. We also ectopically expressed a wild type and catalytically inactive Dnmt2 form in the Dnmt2 mutant background. RNA bisulfite sequencing was used to follow RNA methylation at partical tRNA substrates.
 
Overall design Measuring (cytosine-5) RNA methylation levels in various tRNAs from adult wild type controls, Dnmt2 and NSun2 mutants, and ectopically expressed Dnmt2 in the Dnmt2 null mutant background.
 
Contributor(s) Genenncher B, Schaefer M
Citation(s) 29444437
Submission date Jan 12, 2018
Last update date Feb 20, 2019
Contact name Schaefer Matthias
E-mail(s) matthias.schaefer@meduniwien.ac.at
Organization name Medical University Vienna, Center for Anatomy & Cell Biology
Department Cell and Developmental Biology
Lab Schaefer Lab
Street address Schwarzspanierstrasse 17-I
City Vienna
State/province AT
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (16)
GSM2934969 tRNA-AspGUC in control
GSM2934970 tRNA-GlyGCC in control
GSM2934971 tRNA-MetCAU in control
Relations
BioProject PRJNA430044
SRA SRP130970

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109133_RAW.tar 20.0 Kb (http)(custom) TAR (of TXT)
GSE109133_readme.txt 330 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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