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Series GSE109108 Query DataSets for GSE109108
Status Public on Jan 12, 2018
Title Transcriptome profiling of de-differentiated chondrocytes over-expressing miR-138 or miR-181ab1 during osteogenic induction
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to investigate the effects of miR-138 or miR-181ab1 on regulating osteogenesis. Gene expression profiles were analyzed in human de-differentiated chondrocytes (DDCs) over-expressing either miR-138 or miR-181ab1 following day 2 or day 7 of osteogenic induction. DDCs were isolated from human osteoarthritic articular cartilage and over-expression of miR-138 or miR-181ab1 was induced following lentiviral transduction of DDCs over-expressing the precursor form of miR-138 or miR-181ab1. Control DDCs were transduced with lentivirus expressing a non-silencing (NS) control RNA. Total RNA was harvested at day 2 or day 7 following osteogenic induction and subjected to RNA-Seq analysis.
Overall design Triplicate samples of de-differentiated chondrocytes at P3-4 were transduced with lentivirus expressing either precuror miR-138, precursor miR-181ab1 or lentivirus expressing a non-silencing (NS) control precursor RNA. Transuced cells were then induced to undergo ostegenic differentiation. At days 2 and 7 following osteogenic induction, total RNA was isolated and processed for high throughput RNA sequencing.
Contributor(s) McAlinden A, Zheng H
Citation(s) 30828688
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AR064191 Regulation of Skeletal Development by microRNAs WASHINGTON UNIVERSITY Audrey McAlinden
Submission date Jan 11, 2018
Last update date Mar 12, 2019
Contact name Audrey McAlinden
Phone 314-454-8860
Organization name Washington University
Department Orthopaedic Surgery
Street address 660 South Euclid Avenue
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (18)
GSM2931812 cs128_mir138_day2
GSM2931813 cs128_mir138_day7
GSM2931814 cs128_mir181_day2
BioProject PRJNA429652
SRA SRP128927

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Supplementary file Size Download File type/resource
GSE109108_RAW.tar 63.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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