|
| Status |
Public on Jan 12, 2018 |
| Title |
Transcriptome profiling of de-differentiated chondrocytes over-expressing miR-138 or miR-181ab1 during osteogenic induction |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to investigate the effects of miR-138 or miR-181ab1 on regulating osteogenesis. Gene expression profiles were analyzed in human de-differentiated chondrocytes (DDCs) over-expressing either miR-138 or miR-181ab1 following day 2 or day 7 of osteogenic induction. DDCs were isolated from human osteoarthritic articular cartilage and over-expression of miR-138 or miR-181ab1 was induced following lentiviral transduction of DDCs over-expressing the precursor form of miR-138 or miR-181ab1. Control DDCs were transduced with lentivirus expressing a non-silencing (NS) control RNA. Total RNA was harvested at day 2 or day 7 following osteogenic induction and subjected to RNA-Seq analysis.
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|
| Overall design |
Triplicate samples of de-differentiated chondrocytes at P3-4 were transduced with lentivirus expressing either precuror miR-138, precursor miR-181ab1 or lentivirus expressing a non-silencing (NS) control precursor RNA. Transuced cells were then induced to undergo ostegenic differentiation. At days 2 and 7 following osteogenic induction, total RNA was isolated and processed for high throughput RNA sequencing.
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| |
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| Contributor(s) |
McAlinden A, Zheng H |
| Citation(s) |
30828688 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR064191 |
Regulation of Skeletal Development by microRNAs |
WASHINGTON UNIVERSITY |
Audrey McAlinden |
|
| Submission date |
Jan 11, 2018 |
| Last update date |
Mar 12, 2019 |
| Contact name |
Audrey McAlinden |
| E-mail(s) |
mcalindena@wustl.edu
|
| Phone |
314-454-8860
|
| Organization name |
Washington University
|
| Department |
Orthopaedic Surgery
|
| Street address |
660 South Euclid Avenue
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA429652 |
| SRA |
SRP128927 |
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