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Series GSE109037 Query DataSets for GSE109037
Status Public on Nov 06, 2018
Title 10x Genomics Drop-seq single-cell RNA-seq of isolated Adult human spermatogonia, spermatocytes, spermatids & steady-state spermatogenic cells
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To reveal distinct transcriptomes associated with various spermatogenic cells, including spermatogonial stem cells and all of their subsequent progeny, single-cell transcriptomes from Adult human spermatogonia, StaPut-enriched spermatocytes and spermatids, or unselected steady-state spermatogenic cells were used for Drop-Seq analysis. We used the 10x Genomics Chromium (Drop-Seq) to perform single-cell RNA-seq
Overall design Sorted Adult Human spermatogonia, three replicate preparations of steady-state unselected spermatogenic cells, and isolated adult spermatocytes and spermatids were used for this study. In one experiment, a 1:1 mixture of human and mouse steady-state spermatogenic cells were evaluated together to establish a multiplet rate. Data are from a total of 36,451 cells.
Contributor(s) Hermann BP, Cheng K, Singh A, Roa-de-la-Cruz L, Mutoji KN, Gildersleeve H, Chen I, Mayo M, Westernströer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, McCarrey JR
Citation(s) 30404016
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HD090007 Origin and functional significance of the spermatogonial stem cell transcriptome barcode THE UNIVERSITY OF TEXAS AT SAN ANTONIO Brian Peter Hermann
R01 HD078679 Epimutations in Offspring Produced by Assisted Reproductive Technologies (ART) THE UNIVERSITY OF TEXAS AT SAN ANTONIO John R MCCARREY
Submission date Jan 10, 2018
Last update date Mar 26, 2019
Contact name Brian P. Hermann
Phone 210-458-8047
Organization name University of Texas at San Antonio
Department Department of Biology
Street address 1 UTSA Circle
City San Antonio
State/province TX
ZIP/Postal code 78249
Country USA
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
GPL24496 Illumina HiSeq 3000 (Homo sapiens; Mus musculus)
Samples (11)
GSM2928377 HumanSpermatogonia_17-1
GSM2928378 HumanSpermatogonia_17-2
GSM2928379 HumanSpermatogonia_17-5
BioProject PRJNA429472
SRA SRP128724

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109037_AdultHuman-Spermatids_17-11_barcodes.tsv.gz 17.9 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatids_17-11_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatids_17-11_matrix.mtx.gz 55.5 Mb (ftp)(http) MTX
GSE109037_AdultHuman-Spermatids_Reps1-2_barcodes.tsv.gz 30.8 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatids_Reps1-2_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatids_Reps1-2_matrix.mtx.gz 96.1 Mb (ftp)(http) MTX
GSE109037_AdultHuman-Spermatocytes_17-11_barcodes.tsv.gz 9.4 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatocytes_17-11_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatocytes_17-11_matrix.mtx.gz 36.0 Mb (ftp)(http) MTX
GSE109037_AdultHuman-Spermatocytes_Reps1-2_barcodes.tsv.gz 21.0 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatocytes_Reps1-2_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHuman-Spermatocytes_Reps1-2_matrix.mtx.gz 80.5 Mb (ftp)(http) MTX
GSE109037_AdultHumanSpermatogenesis-reps1-3_filtered_barcodes.tsv.gz 30.7 Kb (ftp)(http) TSV
GSE109037_AdultHumanSpermatogenesis-reps1-3_filteredgenes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHumanSpermatogenesis-reps1-3_filteredmatrix.mtx.gz 97.1 Mb (ftp)(http) MTX
GSE109037_AdultHumanSpermatogonia-reps1-3_filteredbarcodes.tsv.gz 45.9 Kb (ftp)(http) TSV
GSE109037_AdultHumanSpermatogonia-reps1-3_filteredgenes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE109037_AdultHumanSpermatogonia-reps1-3_filteredmatrix.mtx.gz 117.8 Mb (ftp)(http) MTX
GSE109037_RAW.tar 338.2 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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