GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE109035 Query DataSets for GSE109035
Status Public on Jan 11, 2018
Title Proximity of oligodendrocytes remodels astrocytes' transcriptome
Organism Mus musculus
Experiment type Expression profiling by array
Summary We and other groups doumented that astrocytes modulate migration, maturation and myelin sythesis of oligodendrocytes through release of neurotransmitters, cytokins and other signaling molecules. However, much less is known about on how the oligodendrocytes affects the astrocytes. We compared the transcriptome of cortical astrocytes when cultured alone and co-cultured with non-touching immortalized precursor oligodendrocytes (Oli-neu) in insert systems. Experimental data indicate that the oligodendrocyte-conditioning medium has a substantial effect on the the gene expression in astrocytes. Moreover, oligodendendrocyte proximity remodels major astrocyte functional pathways.
Overall design we have used the multiple yellow design in which distinctly labeled samples of biological replicates are cohybridized with the microarray, the similarly labeled distinct conditions were compared and the results averaged for both channels. Thus each raw data file contains raw data for two samples (e.g. C1-2_US83300186_252665513680_S01_GE2_1105_Oct12_1_4.txt contains raw data for both C1 and C2 samples) and dye assignment is indicated in the label field in the corresponding sample records.
Contributor(s) Iacobas DA, Iacobas S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 10, 2018
Last update date Jan 24, 2018
Contact name Dumitru Andrei Iacobas
Phone 936-261-9926
Organization name Prairie View A&M University
Department Center for Computational Systems Biology
Lab Personalized Genomics
Street address Ann Preston St
City Prairie View
State/province TX
ZIP/Postal code 77446
Country USA
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (8)
GSM2928368 cortical astrocytes cultured alone_C1
GSM2928369 cortical astrocytes cultured alone_C2
GSM2928370 cortical astrocytes cultured alone_C3
BioProject PRJNA429458

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109035_C1-2_US83300186_252665513680_S01_GE2_1105_Oct12_1_4.txt.gz 15.2 Mb (ftp)(http) TXT
GSE109035_C3-4_US83300186_252665513680_S01_GE2_1105_Oct12_1_3.txt.gz 15.2 Mb (ftp)(http) TXT
GSE109035_I1-2_US83300186_252665513680_S01_GE2_1105_Oct12_1_2.txt.gz 15.3 Mb (ftp)(http) TXT
GSE109035_I3-4_US83300186_252665513680_S01_GE2_1105_Oct12_1_1.txt.gz 15.3 Mb (ftp)(http) TXT
GSE109035_RAW.tar 6.5 Mb (http)(custom) TAR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap