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Series GSE108972 Query DataSets for GSE108972
Status Public on Jan 31, 2018
Title PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of mast cell signalling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PEST-domain-enriched tyrosine phosphatase (PEP) is a cytoplasmic protein tyrosine phosphatase that regulates immune cell functions, including mast cell functions. Using bone marrow derived mast cells (BMMCs) from PEP+/+ and PEP-/- mice, RNA-seq data showed that dinitrophenol (DNP) - activated PEP-/- BMMCs have misregulated gene expression, with some cytokine/chemokine genes (eg.TNF╬▒, IL13, CSF2) showing reduced gene expression in the dinitrophenol (DNP) - activated PEP-/- BMMCs compared to (DNP)-activated PEP+/+ BMMCs. Also, the ability of the glucocorticoid dexamethasone (Dex) to negatively regulate DNP - induced COX-2 gene expression in PEP-/- BMMCs was inhibited compared to the PEP+/+ BMMCs.
 
Overall design Biological replicates are sequenced and analyzed. The samples are either wild-type or mutant for PEP and cells were sensitized with Ig-E, activated with Dinitrophenol and glucocorticoid treatment done with Dexamethasone.
 
Contributor(s) Ainooson GK, Gourain V, Cato AC
Citation(s) 30238026
Submission date Jan 09, 2018
Last update date Oct 16, 2018
Contact name Victor GOURAIN
Organization name Centre de recherche en transplantation et immunologie
Department UMR1064
Street address 30 Bd Jean Monnet
City Nantes
ZIP/Postal code 44093
Country France
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM2917184 Sample _1
GSM2917185 Sample _2
GSM2917186 Sample _3
Relations
BioProject PRJNA429265
SRA SRP128580

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108972_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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