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Series GSE108925 Query DataSets for GSE108925
Status Public on Jan 09, 2019
Title Abnormalities in Behaviour, Histology and Prefrontal Cortical Gene Expression Profiles Relevant to Schizophrenia in Embryonic Day 17 MAM-Exposed C57BL/6 Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gestational and perinatal disruption of neural development increases the risk of developing schizophrenia (SCZ) later in life. Embryonic day 17 (E17) methylazoxymethanol (MAM) treatment leads to histological, physiological and behavioural abnormalities in post-puberty rats resembling those described in SCZ patients. However, the validity of E17 MAM-exposed mice as a SCZ model has not been explored. Here we treated E17 C57BL/6 mouse dams with various dosages of MAM. We found that this mouse strain was more vulnerable to MAM treatment than rats and there were gender difference in behavioural abnormalities, histological changes and prefrontal cortical gene expression profiles in MAM (7.5 mg/kg)-exposed mice. Both male and female MAM-exposed mice had deficits in prepulse inhibition but were normal in social interaction and novel object recognition tasks. Female MAM-exposed mice were hyperactive in spontaneous locomotion while male mice were normal. Consistently, only female MAM-exposed mice exhibited reduced brain weight, decreased size of prefrontal cortex (PFC) and enlarged lateral ventricles. Transcriptome analysis of the PFC revealed that there were more differentially expressed genes in female MAM-exposed mice than those in male mice. Moreover, expression of Pvalb, Arc and genes in their association networks were downregulated in the PFC of female MAM-exposed mice. These results show that E17 MAM-exposure in C57BL/6 mice leads to behavioural changes that mimic positive symptoms and sensorimotor gating deficits associated with SCZ. MAM-exposed female mice may be used to study gene expression changes, inhibitory neural circuit dysfunction and glutamatergic synaptic plasticity deficits related to SCZ.
 
Overall design Prefrontal cortex mRNA profiles of E17 MAM-exposed mice were generated by deep sequencing, distinguishing gender, in triplicate, using Illumina X-10.
 
Contributor(s) Ye H, Huo C
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Submission date Jan 09, 2018
Last update date Mar 01, 2019
Contact name Chunyue Huo
E-mail(s) huochunyue@126.com
Phone 13146533104
Organization name Capital Medical University
Street address You an men wai Xi tou tiao Road 10
City Beijing
ZIP/Postal code 100069
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM2916076 WT_male_1
GSM2916077 WT_male_2
GSM2916078 WT_male_3
Relations
BioProject PRJNA429174
SRA SRP128506

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Supplementary file Size Download File type/resource
GSE108925_RAW.tar 12.7 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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