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Status |
Public on Feb 13, 2018 |
Title |
Genome-wide mapping of binding sites of the MADS domain protein FRUITFULL in Arabidopis thaliana |
Organism |
Arabidopsis thaliana |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
FUL has a dual role in regulating floral transition and fruit development. We used ChIP-seq to map and compare the FUL binding events in the two different tissues.
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Overall design |
We used a pFUL:FUL-GFP line to conduct chromatin immuno-precipitation experiments with GFP-specific antibodies sampling two different tissue types, inflorescence meristems and pistils/siliques. The IP was followed by library preparation and deep-sequencing (ChIP-seq) to determine the FUL binding sites on a genome-wide scale. Inflorescence meristems were harvested from 4-5 weeks old 35S:AP1-GR ap1 cal pFUL:FUL-GFP lines, while stage 12-16 pistils/siliques were harvested from ful-1 pFUL:FUL-GFP lines. The tissue was fixed for 15-30’ and the immunoprecipitation was performed according to Kaufmann et al., 2010 (doi: 10.1038/ nprot.2009.244.). Experiments were done in two biological replicates with the according input sample as control.
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Contributor(s) |
van Mourik H, Muiño J, Smaczniak C, Bemer M, Chen D, Angenent G, Kaufmann K |
Citation(s) |
36797351 |
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Submission date |
Dec 22, 2017 |
Last update date |
May 22, 2023 |
Contact name |
Dijun Chen |
E-mail(s) |
chendijun2012@gmail.com
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Phone |
030-2093 49744
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Organization name |
Humboldt University
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Department |
Plant Cell and Molecular Biology
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Lab |
Prof. Kaufmann
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Street address |
Philippstr. 13 (House 22)
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City |
Berlin |
ZIP/Postal code |
10115 |
Country |
Germany |
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Platforms (1) |
GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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Samples (6)
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Relations |
BioProject |
PRJNA427320 |
SRA |
SRP127403 |