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| Status |
Public on Dec 30, 2019 |
| Title |
Microbial activities associated with human intestinal mucosal biofilms change mRNAs and microRNAs to promote carcinogenesis in mice [miRNA] |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Disrupted interactions between host and intestinal bacteria are implicated in the development of colorectal cancer (CRC). However, the functional impacts of these inter-kingdom interactions remain poorly defined. To examine this interplay, we performed small RNA sequencing on the stool of from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues. revealed a group of significant differentially expressed miRNAs specific to BT compared to BX associated ApcMin/+;Il10-/- mice and several miRNAs that correlated with bacterial genera abundances. Our findings suggest complex interactions within bacterial communities affecting host-derived miRNA and CRC development.
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| Overall design |
Small RNA sequencing on stool samples from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues.
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| |
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| Contributor(s) |
Sears C, Jobin C |
| Citation(s) |
31937674 |
| |
| Submission date |
Dec 15, 2017 |
| Last update date |
Feb 10, 2020 |
| Contact name |
Christian Jobin |
| Organization name |
University of Florida
|
| Department |
Medicine
|
| Street address |
2033 Mowry Rd, CGRC-461, PO Box: 103633
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE108165 |
Microbial activities associated with human intestinal mucosal biofilms change mRNAs and microRNAs to promote carcinogenesis in mice |
|
| Relations |
| BioProject |
PRJNA422621 |
| SRA |
SRP126874 |
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