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Status |
Public on Sep 27, 2018 |
Title |
Genomic occurrence analysis of PRMT6-dependent H3R2me2a |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Protein arginine methyltransferase 6 (PRMT6) is an epigenetic regulator of fundamental cellular processes, such as gene expression and DNA repair. Asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a) is the major histone modification catalyzed by PRMT6. To identify the genome-wide deposition and transcriptional impact of H3R2me2a, we established PRMT6 deletion in a human cell model of neural differentiation. These knockout cells show severe neural differentiation defects. ChIP-seq profiling reveals that H3R2me2a is present at promoter as well as non-promoter sites in a PRMT6-dependent manner. Loss of H3R2me2a causes enhanced H3K4me3 deposition and target gene transcription supporting a genome-wide repressive nature of H3R2me2a. Intriguingly, the non-promoter H3R2me2a peaks co-localize with active enhancer marks, such as H3K4me1 and H3K27ac.
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Overall design |
Examining of PRMT6-dependent H3R2me2a deposition in CRISPR control and knockout PRMT6 with neighboring histone modification.
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Contributor(s) |
Bouchard C, Sahu P, Nötzold RR, Bauer UM |
Citation(s) |
30232013 |
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Submission date |
Dec 01, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Uta Maria Bauer |
E-mail(s) |
bauer@imt.uni-marburg.de
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Organization name |
Philipps University Marburg
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Department |
Institute of Molecular Biology and Tumor Research
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Lab |
AG Bauer
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Street address |
Hans-Meerwein-Str. 2
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City |
Marburg |
State/province |
Hessen |
ZIP/Postal code |
35043 |
Country |
Germany |
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Platforms (1) |
GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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Samples (22)
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This SubSeries is part of SuperSeries: |
GSE107612 |
Genomic location of PRMT6-dependent H3R2 methylation is decisive for the transcriptional outcome of associated genes |
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Relations |
BioProject |
PRJNA420759 |
SRA |
SRP125974 |