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Series GSE107586 Query DataSets for GSE107586
Status Public on Dec 06, 2018
Title Profiling the transcriptome of layer 5 intratelencephalic-projection neurons (IT-PNs) in the mature mouse motor cortex
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Low input RNA-seq analysis of fluorescently activated cell sorted IT-PNs from layer 5 of the mature mouse motor cortex. Comparison of two distinct IT-PN subtypes previously described by Tantirigama et al. (2014) identifies unique gene expression.
 
Overall design A Fezf2-Gfp reporter mouse model was injected at postnatal day 23 - 25 with the retrograde tracer, cholera toxin subunit b conjugated to Alexa 647, in M1 of the left hemisphere (n = 5). Five to seven days after injection, the layer 5 of the right hemisphere M1 was dissected and dissociated for FACS-purification of Fezf2+ and Fezf2- IT-PNs. A novel approach to low-input RNA-seq was used to generate cDNA libraries before sequencing.
 
Contributor(s) Clare AJ, Day RC, Empson RM, Hughes SM
Citation(s) 30483051
Submission date Dec 01, 2017
Last update date May 15, 2019
Contact name Alison Jane Clare
E-mail(s) alisonjclare@hotmail.co.uk
Organization name University of Otago
Department Biochemistry
Lab Neurodegenerative and Lysosome Disease Lab
Street address 710 Cumberland Street
City Dunedin
State/province Otago
ZIP/Postal code 9016
Country New Zealand
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (10)
GSM2871713 Fezf2+ IT-PN (1)
GSM2871714 Fezf2+ IT-PN (2)
GSM2871715 Fezf2+ IT-PN (3)
Relations
BioProject PRJNA420717
SRA SRP126001

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Supplementary file Size Download File type/resource
GSE107586_DESeq2_analysis.txt.gz 245.8 Kb (ftp)(http) TXT
GSE107586_Raw_Counts.txt.gz 149.6 Kb (ftp)(http) TXT
GSE107586_Raw_Counts_ERCC.txt.gz 1.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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