GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE107152 Query DataSets for GSE107152
Status Public on Mar 13, 2020
Title Haematopoetic RNA-seq from ultra low input
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The correct balance between self-renewal and differentiation of hematopoietic stem cells (HSC) is orchestrated by the crosstalk between extrinsic and intrinsic factors. A combination of transcriptional regulators and chromatin-based epigenetic changes provide a fine-tuning mechanism to determine HSC fate. We report that the histone H4 lysine 16 (H4K16) specific lysine acetyl-transferase MOF (KAT8) shows dynamic chromatin occupancy during HSC differentiation. MOF regulates erythroid commitment by regulating the expression of key haematopoietic genes. Indeed, loss of just one copy of Mof perturbs HSC commitment, leading to skewed granulocytic lineage commitment and impaired erythroid maturation in mice. Wild-type MOF and strikingly, enforced expression of the transcription factor GATA-1, rescued hematopoietic skewing of this granulocytic fate bias. Furthermore, single-cell RNA-seq of Mof haploinsufficient HSCs revealed an enrichment of a novel immature heterogeneous sub-population of cells with increased proliferation potential, indicative of an enhanced pro-leukemic state. Therefore, our results demonstrate an unprecedented role of MOF in the regulation of HSC plasticity, identity and differentiation.
Overall design 15 cells from 6 different progenitor cells: HSCs (LSK+CD34-Flt3-CD48-CD150+), MPP2 (LSK+CD34+Flt3-CD48+CD150+), MPP3 (LSK+CD34+Flt3-CD48+CD150-), MPP4 LSK+CD34+Flt3+CD48+CD150-), CMP Lin-Sca-1-cKit+CD34+IL7r-CD16/32low) and MEP (Linegae-Sca-1-cKit+CD34-IL7r-CD16/32-) were sorted by Aria FACS Fusion II (BD)
Web link
Contributor(s) Rodrigures CP, Herman JS, Grun D, Akhtar A
Citation(s) 32671208
Submission date Nov 20, 2017
Last update date Jul 20, 2020
Contact name Asifa Akhtar
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Chromatin Regulation
Lab Akhtar Lab
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2861739 Early progenitor wt (contain 3 rep)
GSM2861740 Early progenitor mut (contain 3 rep)
GSM2861741 MPPs wt (contain 3 rep)
This SubSeries is part of SuperSeries:
GSE107154 Decoding the regulatory circuit by epigenetic regulator MOF in haematopoiesis
BioProject PRJNA419186
SRA SRP125307

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107152_15cells_barcode.xlsx 51.7 Kb (ftp)(http) XLSX
GSE107152_WT_CMP.txt.gz 140.0 Kb (ftp)(http) TXT
GSE107152_WT_HSC.txt.gz 128.8 Kb (ftp)(http) TXT
GSE107152_WT_MEP.txt.gz 138.0 Kb (ftp)(http) TXT
GSE107152_WT_MPP2.txt.gz 113.6 Kb (ftp)(http) TXT
GSE107152_WT_MPP3.txt.gz 112.9 Kb (ftp)(http) TXT
GSE107152_WT_MPP4.txt.gz 128.8 Kb (ftp)(http) TXT
GSE107152_het_CMP.txt.gz 144.6 Kb (ftp)(http) TXT
GSE107152_het_HSC.txt.gz 120.5 Kb (ftp)(http) TXT
GSE107152_het_MEP.txt.gz 140.9 Kb (ftp)(http) TXT
GSE107152_het_MPP2.txt.gz 125.1 Kb (ftp)(http) TXT
GSE107152_het_MPP3.txt.gz 142.3 Kb (ftp)(http) TXT
GSE107152_het_MPP4.txt.gz 141.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap