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Status |
Public on Jul 26, 2019 |
Title |
Expression profile of lncRNAs and mRNAs in 4 brain regions of adult mouse |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array Non-coding RNA profiling by array
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Summary |
We aimed to decipher the spatial expression landscape of well-annotated lncRNAs in brain regions. To this end, we dissected adult male mice brain tissues from olfactory bulb (OB), hypothalamus (Hypo), hippocampus (Hippo) and cerebellum (Cereb) and subjected to Arraystar Mouse LncRNA Microarray V3.0. We found a new type of genome organization formed by highly conserved lncRNA cluster and nearby TF in the non-imprinting regions that is associated with human CNS disorders.
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Overall design |
10-week-old male BALB/c mice (Vital River Laboratories, Beijing, China) were used in this study and the mice were housed in a temperature-controlled room under a 12 hours light/12 hours dark cycle, with free access to food and water ad libitum. Three mice were used for microarray analysis, and three to ten mice were used for validation. The mice were euthanized by cervical dislocation and brains were carefully dissected using stereomicroscope to separate the four experimental brain regions (olfactory bulb, hypothalamus, hippocampus as well as cerebellum) according to mouse brain atlas (26).
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Contributor(s) |
Wang F, Ren D, Liang X, Ke S, Zhang B, Hu B, Song X, Wang X |
Citation(s) |
31127312 |
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Submission date |
Nov 13, 2017 |
Last update date |
Jul 26, 2019 |
Contact name |
xiaoyuan song |
E-mail(s) |
songxy5@ustc.edu.cn
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Phone |
86-551-63607752
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Organization name |
University of Science & Technology of China
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Street address |
Huangshan Road
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City |
Hefei |
ZIP/Postal code |
230027 |
Country |
China |
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Platforms (1) |
GPL19286 |
Arraystar Mouse LncRNA microarray V3B (Agilent-055750 custom Probe Name version) |
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Samples (12)
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Relations |
BioProject |
PRJNA418153 |