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Series GSE106465 Query DataSets for GSE106465
Status Public on Nov 03, 2017
Title Massive and reproducible production of liver buds entirely from human pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Organoid technology provides a revolutionary paradigm towards therapy, yet to be applied in humans mainly because of the reproducibility and scalability challenges. Here, we overcome these limitations by evolving scalable organ bud production platform entirely from human induced pluripotent stem cells (iPSC). By conducting massive ‘reverse’ screen experiments, we identified effective triple progenitor populations for generating liver buds in a highly reproducible manner: hepatic endoderm, endothelial and septum mesenchyme progenitors. Furthermore, we achieved human scalability by developing an omni-well-array culture platform for mass-producing homogenous and miniaturized liver buds on a clinically relevant large scale (>108-cell scale). Vascularized and functional liver tissues generated entirely from iPSC significantly improved subsequent hepatic functionalization potentiated by stage-matched developmental progenitor interactions, enabling functional rescue against acute liver failure via transplantation. Overall, our study provides a stringent manufacture platform for multi-cellular organoid supply, thus facilitating clinical and pharmaceutical applications especially for the treatment of liver diseases through multi-industrial collaborations.
 
Overall design Transcriptome profiling of iPSC derived liver buds (iPSC-LB), progenitor populations for generating liver buds (iPSC-tHE/iPSC-EC/iPSC-MC) and human liver tissue of various developmental stages.
 
Contributor(s) Takebe T, Ueno Y, Koido M, Taniguchi H
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Submission date Nov 02, 2017
Last update date Jan 24, 2018
Contact name Yasuharu Ueno
E-mail(s) uenoy@yokohama-cu.ac.jp
Phone +81-45-787-8963
Fax +81-45-787-8963
Organization name Yokohama City University
Department Graduate School of Medicine
Lab Department of Regenerative Medicine
Street address 3-9 Fuku-ura, Kanazawa-ku
City Yokohama City
State/province Kanagawa
ZIP/Postal code 236-0004
Country Japan
 
Platforms (2)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (61)
GSM2837984 iPS_DE_d6_1
GSM2837985 iPS_DE_d6_2
GSM2837986 iPS_DE_d6_3
Relations
BioProject PRJNA416927

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106465_RAW.tar 180.7 Mb (http)(custom) TAR (of TXT)
GSE106465_matrix.xls.gz 25.5 Mb (ftp)(http) XLS
Processed data are available on Series record

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