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Series GSE106315 Query DataSets for GSE106315
Status Public on Apr 18, 2018
Title Srf destabilizes cell identity (RNA-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Multicellular organisms consist of multiple cell types, whose identities are maintained appropriately at locations where they are reside. The identity of each cell type is primarily maintained by cell-type-specific gene expression programs, but mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease β-actin monomer resulted in nuclear accumulation of Mkl1 and the activation of Srf, which downregulated cell-type-specific genes and altered epigenetics in enhancers and chromatin organization. Mice overexpressing Srf exhibited various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
 
Overall design Gene expressions comparing cells cultured on different stiffnesses
 
Contributor(s) Ikeda T, Hikichi T, Yamamoto T, Okita K, Masui S
Citation(s) 29643333
Submission date Oct 30, 2017
Last update date May 15, 2019
Contact name Takashi Ikeda
E-mail(s) tikeda@cira.kyoto-u.ac.jp
Organization name Kyoto Univ.
Street address 53 Shogoin Kawahara-cho Sakyo-ku
City Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2835855 RNA-seq_NPC_control_0.5kPa
GSM2835856 RNA-seq_NPC_control_12kPa
GSM2835857 RNA-seq_NPC_shSrf_0.5kPa
This SubSeries is part of SuperSeries:
GSE90034 Srf destabilizes cell identity
Relations
BioProject PRJNA416280
SRA SRP122908

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106315_RAW.tar 610.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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