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Status |
Public on Dec 16, 2018 |
Title |
Agilent 8x60K Custom Exon array of human temporal cortical tissue samples with varying degree of Alzheimer's Disease (AD)-related neurofibrillary pathology |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
In this study, we coupled microarray-based transcriptomics and MS-based phosphoproteomics assay to determine mRNA, protein, and phosphopeptide expression levels from 71 autopsied temporal cortical samples, with varying degree of Alzheimer's Disease (AD)-related neurofibrillary pathology. With computational analysis, we identified disease-related transcript, protein and phosphopeptide expression patterns, associated with distinct biological processes and cell types.
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Overall design |
71 human temporal cortical tissue samples were divided into 7 groups based on Braak staging (degree of AD-related neurofibrillary pathology) indicating the degree of disease severity. From 60 of these samples, transcripts were identified by microarray analysis. Additionally, utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS) phospho-proteomics, proteins and phosphopeptides were identified from 36 samples (Data found in PRIDE). Furthermore, APOE genotype, Alpha-, beta-, and gamma-secretase activity, and amyloid-beta 42 levels were determined from the tissue samples.
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Contributor(s) |
Marttinen M, Paananen J |
Citation(s) |
30557660 |
Submission date |
Oct 26, 2017 |
Last update date |
Mar 22, 2019 |
Contact name |
Mikael Marttinen |
E-mail(s) |
mikael.marttinen@uef.fi
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Organization name |
University of Eastern Finland
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Department |
Biomedicine
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Street address |
Yliopistonranta 1E
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City |
Kuopio |
ZIP/Postal code |
70210 |
Country |
Finland |
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Platforms (1) |
GPL24170 |
Agilent-044312 Human 8x60K Custom Exon array (Probe Name version) |
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Samples (60)
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Relations |
BioProject |
PRJNA415984 |
Supplementary file |
Size |
Download |
File type/resource |
GSE106241_Data_matrix_full.csv.gz |
11.3 Mb |
(ftp)(http) |
CSV |
GSE106241_RAW.tar |
140.7 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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