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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 03, 2018 |
| Title |
Gene expression changes upon activation of RIG-I by a minimal potent RIG-I ligand |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have developed highly potent synthetic activators of the vertebrate immune system that specifically target the RIG-I receptor. When introduced into mice, a family of short, triphosphorylated Stem Loop RNAs (SLRs) induces a potent interferon response and the activation of specific genes essential for antiviral defense. Using RNAseq, we provide the first in-vivo genome-wide view of the expression networks that are initiated upon RIG-I activation. We observe that SLRs specifically induce type I interferons, subsets of interferon-stimulated genes (ISGs), and cellular remodeling factors. By contrast, poly(I:C), which binds and activates multiple RNA sensors, induces type III interferons and several unique ISGs. The short length (10-14 base pairs) and robust function of SLRs in mice demonstrate that RIG-I forms active signaling complexes without oligomerizing on RNA. These findings demonstrate that SLRs are potent therapeutic and investigative tools for targeted modulation of the innate immune system.
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| Overall design |
We have triplicate samples of spleens from mice treated with SLR10, SLR14, poly(I:C) and pppNS, we have duplicate samples of mice treated with jetPEI vehicle control, and we have triplicate samples of untreated control mice
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| Contributor(s) |
Dickey TH, Molinari ES, Linehan MM, Fitzgerald ME, Potapova O, Iwasaki A, Pyle AM |
| Citation(s) |
29492454 |
| Submission date |
Oct 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Anna Marie Pyle |
| E-mail(s) |
anna.pyle@yale.edu
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| Organization name |
Yale University
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| Street address |
219 Prospect St.
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA418284 |
| SRA |
SRP124941 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106229_EdgeR_normalized_CPMs.csv.gz |
1.7 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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