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Series GSE106199 Query DataSets for GSE106199
Status Public on Dec 20, 2017
Title MicroRNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary (HN1L) is a targetable breast cancer stem cell (BCSC) gene that is altered in 25% of whole breast cancer and significantly correlated with shorter overall or relapse-free survival in triple negative breast cancer (TNBC) patients. HN1L silencing reduced the population of BCSCs, inhibited tumor initiation, re-sensitized chemo-resistant tumors to docetaxel, and hindered cancer progression in multiple TNBC cell line derived xenografts.
We used miRNA array to compare miRNA expression profiles from PDX BCM2665 xenograft tumors with or without HN1L knockdown, to identify role of HN1L in regulating miRNA.
Overall design miRNA arrays were performed on 10 snap-frozen tumor samples from scrambled siRNA-treated and HN1L siRNA-treated BCM2665 xenografts (5 samples from each treatment group). Differencial expression was performed to identifiy significantly differencially expressed miRNA and corresponding pathways.
Contributor(s) Chang JC, Elemento O, Verma A, Liu Y, Choi DS, Wong H
Citation(s) 29249663
Submission date Oct 26, 2017
Last update date Mar 21, 2018
Contact name Jenny Chang
Organization name Houston Methodist Hospital
Department Houston Methodist Cancer Center
Street address 6670 Bertner Ave.
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (10)
GSM2831501 tumor from scrambled siRNA-treated mouse #4817 [miRNA]
GSM2831502 tumor from scrambled siRNA-treated mouse #4819 [miRNA]
GSM2831503 tumor from scrambled siRNA-treated mouse #4959 [miRNA]
This SubSeries is part of SuperSeries:
GSE106200 RNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples
BioProject PRJNA415910

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106199.txt.gz 2.0 Kb (ftp)(http) TXT
GSE106199_RAW.tar 8.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

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