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Series GSE106105 Query DataSets for GSE106105
Status Public on Oct 25, 2017
Title PGAM5 promotes lasting FoxO activation after developmental mitochondrial stress and extends lifespan in Drosophila
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary The mitochondrial unfolded protein response (UPRmt) has been associated with long lifespan across metazoans. In Caenorhabditis elegans, mild developmental mitochondrial stress activates UPRmt reporters and extends lifespan. We show that similar developmental stress is necessary and sufficient to extend Drosophila lifespan, and identify Phosphoglycerate Mutase 5 (PGAM5) as a mediator of this response. Developmental mitochondrial stress leads to activation of FoxO, via Apoptosis Signal-regulating Kinase 1 (ASK1) and Jun-N-terminal Kinase (JNK). This activation persists into adulthood and induces a select set of chaperones, many of which have been implicated in lifespan extension in flies. Persistent FoxO activation can be reversed by a high- protein diet in adulthood, through mTORC1 and GCN-2 activity. Accordingly, the observed lifespan extension is prevented on a high-protein diet and in FoxO-null flies. The diet-sensitivity of this pathway has important implications for interventions that seek to engage the UPRmt to improve metabolic health and longevity.
Overall design Three thoracic samples, including one genetic control and two experimental conditions. Also eight fat body samples, four each from adults and larvae, from wild type and PGAM5 mutant flies, with and without RU486 treatment to induce genetic phenotype.
Contributor(s) Borch Jensen M, Qi Y, Riley RR, Rabkina L, Jasper H
Citation(s) 28891792
Submission date Oct 24, 2017
Last update date May 15, 2019
Contact name Martin Borch Jensen
Organization name Buck Institute for Research on Aging
Lab Jasper
Street address 8001 Redwood Blvd.
City Novato
State/province California
ZIP/Postal code 94945
Country USA
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (11)
GSM2829781 thorax_control
GSM2829782 thorax_ND75
GSM2829783 thorax_dOTC
BioProject PRJNA415612
SRA SRP121315

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Supplementary file Size Download File type/resource
GSE106105_processed_data.xlsx 2.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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