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Series GSE105906 Query DataSets for GSE105906
Status Public on May 31, 2018
Title Comparative transcriptome analysis of murine macrophages infected with Brucella suis vaccine strain S2 and the virulent Brucella suis strain 1330
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Brucellosis is one of the most common zoonotic epidemics worldwide. Vaccination against Brucellosis is an important control strategy to prevent the disease in many high-prevalence regions. At present, Brucella vaccine strain S2 is the most widely used vaccine in China. In this study, to uncover the related mechanisms underlie virulence attenuation of S2, we characterized the transcriptional profile of S2 and 1330 infected macrophages by transcriptome analysis. The results revealed that expressions of 440 genes were significantly different between macrophages infected by 1330 and S2. Data analysis showed that in the gene ontology term, the different expressed genes involved in innate immune response, phagoctyosis, recognition, and inflammatory response were significantly enriched. Pathway enrichment analysis indicated that the genes involved in transcriptional misregulation in cancer, staphylococcus aureus infection pathways and NF-kappa B signaling pathway were significantly affected. To reveal the molecular mechanisms related to different expression profiles of infected macrophages, the transcription levels of the different genes between the two bacterial genomes were also detected. In total, the transcription of 29 different genes was significantly changed in either culture medium or infected microphages. The results of current study can be conducive to the promotion of better understanding of the related mechanisms underlie virulence attenuation of S2 and interactions between host cells and brucella strains.
Overall design Two groups were analyzed, and each group contained 3 replicates.
Contributor(s) Jiang H, Dong H, Zhu L, Peng X, Feng Y, Peng Y, Ding J, Fan H
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Submission date Oct 23, 2017
Last update date May 15, 2019
Contact name Hui Jiang
Phone 15011216921
Organization name China Insititute of Veterinary Drug Control
Street address NO.8 Zhonguancun South Street
City Beijing
ZIP/Postal code 100081
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM2828675 S2 rep1
GSM2828676 S2 rep2
GSM2828677 S2 rep3
BioProject PRJNA415479
SRA SRP121014

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Supplementary file Size Download File type/resource
GSE105906_all.counts.txt.gz 312.7 Kb (ftp)(http) TXT
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