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Series GSE1059 Query DataSets for GSE1059
Status Public on Feb 21, 2004
Title Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS, and stably-transfected with the pcDNA3.1/V5-His TOPO vector (Invitrogen) harbouring either CREB, CREM alpha, CREM tau2alpha, ATF2, ATF2-small gene or a control empty-vector. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization.
Keywords: other
Contributor(s) Bailey J, Europe-Finner GN
Citation(s) 15691874
Submission date Feb 20, 2004
Last update date Aug 10, 2018
Contact name Jarrod Bailey
Phone (+44) 191 2228748
Fax (+44) 191 2225066
Organization name University of Newcastle upon Tyne
Department School of Surgical and Reproductive Sciences
Street address Leech Building, Medical School
City Newcastle upon Tyne
State/province Tyne and Wear
ZIP/Postal code NE2 4HH
Country United Kingdom
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (18)
GSM17039 Control human myometrial cells
GSM17040 Control empty-vector stably-transfected human myometrial cells
GSM17041 Control empty-vector stably-transfected human myometrial cells (replicate)
BioProject PRJNA87139

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