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Series GSE105768 Query DataSets for GSE105768
Status Public on Feb 07, 2018
Title Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-seq2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we were performed RNAseq to analyze the transcriptome changing of Hoxb5 in Pro/pre-B by over expression.
 
Overall design B220+ enriched cells from bone marrow were used to transduce Hoxb5 virus or GFP control virus. And 1000 transduced Pro/pre-B cells were sorted for RANseq analysis. The cDNA of sorted 1000-cell aliquots were generated and amplified as described previously (PMID;20203668). Then quality controls of the amplified cDNA were examined by qPCR analysis of housekeeping genes (B2m, Actb, Gapdh, Ecf1a1). RNAseq libraries of 1000 cells were generated by Nextera XT DNA Sample Preparation Kit (FC-131-1096) with 1 ng of generated dsDNA following the manufacturer’s protocol. The samples passed quality control were used for sequencing library preparation by illumine Nextera XT DNA Sample Preparation Kit (FC-131-1096). All libraries were sequenced by illumina sequencers NextSeq 500 (illumina).
 
Contributor(s) Dong Y, Zhang M, Wang J
Citation(s) 29434353
Submission date Oct 22, 2017
Last update date May 15, 2019
Contact name Yong Dong
E-mail(s) dongyong@cmc.edu.cn
Organization name Chengdu medical college
Department Immunology
Street address Xindu road 783, Xindu district
City chengdu
State/province sichuan
ZIP/Postal code 610500
Country China
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (16)
GSM2827696 control_Pro/pre-B_rep1
GSM2827697 control_Pro/pre-B_rep2
GSM2827698 control_Pro/pre-B_rep3
This SubSeries is part of SuperSeries:
GSE105057 Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes
Relations
BioProject PRJNA415304
SRA SRP120856

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Supplementary file Size Download File type/resource
GSE105768_genes_fpkm_table.csv.gz 1.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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