Expression profiling by high throughput sequencing
Summary
Here, we performed RNA-Seq on mRNA isolated from primary dermal fibroblasts of human, mice and multiple non-human primate species transfected with polyinosinic-polycytidylic acid (polyI:C), a synthetic dsRNA analog that invokes a strong interferon-mediated response, or mock transfected.
Overall design
For each of the ten species, primary fibroblasts were acquired from three donors. Cells from each donor were transfected in triplicate with 0.5 ug poly(I:C)/well in a six well plate (~0.05 ug/cm2) or mock-transfected (all transfection mixture components minus poly(I:C)) in triplicate. Thus, there were a total of 180 samples (10 species x 3 donors x 6 samples per donor).