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| Status |
Public on Jan 08, 2018 |
| Title |
Simple and Complex Centromeric Satellites in Drosophila Sibling Species |
| Organisms |
Drosophila melanogaster; Drosophila simulans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Centromeres are the chromosomal sites of assembly for kinetochores, the protein complexes that attach to spindle fibers and mediate separation of chromosomes to daughter cells in mitosis and meiosis. In most multicellular organisms, centromeres comprise a single specific family of tandem repeats, often 100-400 bp in length, found on every chromosome, typically in one location within heterochromatin. Drosophila melanogaster is unusual in that the heterochromatin contains many families of mostly short (5-12 bp) tandem repeats, none of which appears to be present at all centromeres, and none of which is found only at centromeres. Although centromere sequences from a minichromosome have been identified and candidate centromere sequences have been proposed, the DNA sequences at native Drosophila centromeres remain unknown. Here we use native chromatin immunoprecipitation to identify the centromeric sequences bound by the foundational kinetochore protein cenH3, known in vertebrates as CENP-A. In D. melanogaster, these sequences include a few families of 5-bp and 10-bp repeats, but in closely related D. simulans, a partially overlapping set of short repeats and more complex repeats comprise the centromeres. The results suggest that a recent expansion of short repeats is replacing more complex centromeric repeats in the melanogaster subgroup of Drosophila.
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| Overall design |
We used native chromatin immunoprecipitation with anti-CENP-A antibodies to enrich for and sequence centromeric satellites bound by CENP-A in two sibling Drosophila species. We counted 71 candidate repeated sequences and their reverse complements to determine which were enriched in the immunoprecipitates.
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| Contributor(s) |
Talbert P, Kasinathan S, Henikoff S |
| Citation(s) |
29305387 |
| |
| Submission date |
Oct 17, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jorja Henikoff |
| E-mail(s) |
jorja@fhcrc.org
|
| Phone |
206-667-4850
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Basic Sciences
|
| Lab |
Henikoff
|
| Street address |
1100 Fairview AV N, A1-162
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
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| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL22293 |
Illumina HiSeq 2500 (Drosophila simulans) |
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| Samples (19)
|
| GSM2817931 |
CidGFP_GFP_5m_IN_(20160810_PT_DmSc_CG_IN_71216) |
| GSM2817932 |
CidGFP_GFP_5m_IP_(20160810_PT_DmSc_CG_IP_71216) |
| GSM2817933 |
Dmel_CidH_5m_IN_(20170502_PT_DmSc_Cid8_in0418) |
| GSM2817934 |
Dmel_CidH_5m_IP_(20170502_PT_DmSc_Cid8_IP0418) |
| GSM2817935 |
Dmel_CidM_15m_IN_long_(20151015_PT_Dm_15m_IN_0720) |
| GSM2817936 |
Dmel_CidM_15m_IN_(20150818_PT_Dm_15m_IN_0720) |
| GSM2817937 |
Dmel_CidM_15m_IP_long_(20151015_PT_Dm_15m_IP_0720) |
| GSM2817938 |
Dmel_CidM_15m_IP_(20150818_PT_Dm_15m_IP_0720) |
| GSM2817939 |
Dmel_CidM_5m_IN_(20160125_PT_Dm_5min_In_0720) |
| GSM2817940 |
Dmel_CidM_5m_IP_(20160125_PT_Dm_5min_IP_0720) |
| GSM2817941 |
Dsim_CidM_10m_long_(20151015_PT_Ds_ML82_10m_input) |
| GSM2817942 |
Dsim_CidM_15m_IN_(20151207_PT_Ds_ML82_In_15min) |
| GSM2817943 |
Dsim_CidM_15m_IP_(20151207_PT_Ds_ML82_IP_15min) |
| GSM2817944 |
Dsim_CidM_5m_IN2_(20170622_PT_Ds_ML82_5mIn0530) |
| GSM2817945 |
Dsim_CidM_5m_IN_(20151207_PT_Ds_ML82_In_5min) |
| GSM2817946 |
Dsim_CidM_5m_IP2_(20170622_PT_Ds_ML82_5mIP0530) |
| GSM2817947 |
Dsim_CidM_5m_IP_(20151207_PT_Ds_ML82_IP_5min) |
| GSM2817948 |
OreR_GFP_5m_IN_(20160810_PT_DmSc_OR_IN_71216) |
| GSM2817949 |
OreR_GFP_5m_IP_(20160810_PT_DmSc_OR_IP_71216) |
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| Relations |
| BioProject |
PRJNA414639 |
| SRA |
SRP120186 |
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