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Series GSE104895 Query DataSets for GSE104895
Status Public on Jan 01, 2018
Title Genome-wide profiling of miRNA associated with BCR-FGFR1 fusion kinase driven stem cell leukemia and lymphoma II
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The BBC1 and BBC2 cell lines were isolated from pre-B-cell lymphoma models of a murine BCR-FGFR1 driven SCLL. miRNA profiles for BBC2 cells, were established using miRNA arrays. First, BBC2 cells were compared with FACS sorted, normal, murine splenic CD19+ B cells isolated from BALB/c mice, where upregulated and downregulated miRNAs in BBC2 cells were identified. We next investigated which of these miRNAs were affected by loss of FGFR1 function. Previously we showed that the FGFR inhibitor BGJ398 suppresses suppressed phosphoactivation of chimeric FGFR1 kinases. When BBC2 cells were treated with 50nM BGJ398 for 24 hours, up- and down-regulated miRNAs by FGFR1 signaling were identified. By comparasion of the two subsets of differentially expressed miRNAs, we were able to define core candidate miRNAs which appear to be regulated by BCR-FGFR1 in SCLL.
Overall design The miRNA profiles of BBC2 cell sample treated with DMSO and BGJ398 were detected with miRNA microarray.
Contributor(s) Cowell JK, Hu T
Citation(s) 29367757
Submission date Oct 12, 2017
Last update date Apr 02, 2018
Contact name John Cowell
Phone 7067214381
Organization name Augusta University
Street address 1410 Laney-Walker Blvd
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (4)
GSM2809681 BBC2_BGJ50-1
GSM2809682 BBC2_BGJ50-2
GSM2809683 BBC2_DMSO-1
BioProject PRJNA414101

Download family Format
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Supplementary data files not provided
Processed data included within Sample table

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